2026-10-12T13:24:19Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34002807 2026-09-26T11:56:46Z category_1 category_4 category_7 category_12 category_14 category_19 category_873 category_811 category_64 category_133 portal_87 item_type_3 month_year_09_2026

Cisplatin combined with high-intensity focused ultrasound inhibits endometrial cancer progression by inducing pyroptosis Chuqiang Shu (12997731) Pu Zhang (51027) Qi Tian (123361) Ziqing Wan (17694710) Guang Li (109360) Chengzhi Li (4398325) Zhibiao Wang (524160) Biophysics Biochemistry Medicine Cell Biology Molecular Biology Pharmacology Chemical Sciences not elsewhere classified Mathematical Sciences not elsewhere classified Cancer Plant Biology Endometrial cancer cisplatin high-intensity focused ultrasound (HIFU) pyroptosis PI3K/AKT pathway <p>Cisplatin-based chemotherapy is limited by toxicity and resistance.High-intensity focused ultrasound (HIFU) is a noninvasive ablation technique with potential to enhance chemotherapy efficacy.</p> <p>To investigate the anti-tumor effects and mechanisms of cisplatin combined with HIFU in endometrial cancer.</p> <p>Safe cisplatin concentrations and HIFU parameters were screened in HEEpiC-SV40 cells. Proliferation, apoptosis, migration, and invasion of Ishikawa cells were assessed using CCK-8, EdU, colony formation, TUNEL, wound healing, and Transwell assays. Pyroptosis-related markers and PI3K/AKT pathway activity were evaluated by Western blot, qRT-PCR, and ELISA. Therapeutic efficacy was further examined in a nude mouse xenograft model.</p> <p>Cisplatin (5 μg/mL) combined with HIFU (1 MHz, 160 J) was identified as the optimal treatment condition. The combination significantly inhibited Ishikawa cell proliferation, migration, and invasion while promoting pyroptosis. Mechanistically, combined treatment suppressed PI3K/AKT pathway activation and increased pyroptosis-related markers. These effects were reversed by the pyroptosis inhibitor AZL-N. Moreover, PI3K/AKT activation with 740 Y-P attenuated the anti-tumor and pyroptosis-inducing effects of the combination. <i>In vivo</i>, cisplatin combined with HIFU markedly restrained xenograft tumor growth and promoted tumor pyroptosis, whereas 740 Y-P pretreatment counteracted these effects.</p> <p>Cisplatin combined with HIFU suppresses Type I endometrial cancer progression by inhibiting PI3K/AKT signaling and promoting pyroptosis, supporting its potential as a novel combination therapeutic strategy.</p> 2026-09-26T11:56:46Z Dataset Dataset 10.6084/m9.figshare.34002807.v1 https://figshare.com/articles/dataset/Cisplatin_combined_with_high-intensity_focused_ultrasound_inhibits_endometrial_cancer_progression_by_inducing_pyroptosis/34002807 CC BY 4.0