2026-10-11T18:38:08Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33999864 2026-09-25T17:46:32Z category_4 category_7 category_12 category_21 category_873 category_46 category_135 portal_5 item_type_3 month_year_09_2026

<p>Data PLOS.</p> Leonardo Bohorquez Santiago (25111623) Diana Marcela Devia Narváez (25111626) Rogelio Ospina Ospina (25111629) Biochemistry Medicine Cell Biology Biotechnology Chemical Sciences not elsewhere classified Immunology Computational Biology proposed protocol comprises potential bioactive properties localized dopant source following key elements chamber vacuum conditions targeted characterization techniques encompassing laser characteristics uniform thin films based thin films metallic copper filaments ensure effective doping embedding copper filaments div >< p targets :</ b thin films physicochemical characterization laser ablation ensure reproducibility copper doping work provides systematic approach substrate distance stepwise procedure resulting coatings reproducible route reliable framework physically modified papld system papld ). novel approach film growth exhaustive physicochemical details regarding cu films copper species controlled co configuration enables compositional consistency complementary publication biological evaluations biological assessment <div><p>This work provides a detailed and reproducible methodology for the fabrication of copper-doped hydroxyapatite (HAp-Cu) thin films on silicon substrates via Plasma-Assisted Pulsed Laser Deposition (PAPLD). The methodology is centered on the nanosecond pulsed laser ablation of a synthetic hydroxyapatite (HAp) target, physically modified through the incorporation of metallic copper filaments, which serve as a localized dopant source during the deposition process. This configuration enables the controlled co-deposition of hydroxyapatite and copper species, facilitating the formation of uniform thin films with potential bioactive properties. The proposed protocol comprises the following key elements: • <b>Preparation of Targets:</b> A comprehensive, stepwise procedure for the synthesis of hydroxyapatite powder and the subsequent fabrication of mechanically robust ceramic targets, including a novel approach for embedding copper filaments to ensure effective doping during laser ablation. • <b>Deposition Parameters:</b> Detailed specifications of the PAPLD system, encompassing laser characteristics (wavelength, fluence, repetition rate), target-to-substrate distance, and chamber vacuum conditions. These parameters have been optimized to ensure reproducibility in film growth and compositional consistency. • <b>Validation Procedure:</b> A systematic approach to validating the deposition process, focused on confirming the incorporation of copper into the hydroxyapatite matrix and verifying the formation of crystalline HAp-Cu films through targeted characterization techniques, distinct from exhaustive physicochemical or biological evaluations. The methodology described herein establishes a reliable framework for the fabrication of HAp-Cu coatings via PAPLD, offering a reproducible route for future studies requiring precise control over dopant incorporation within hydroxyapatite-based thin films. For further details regarding the physicochemical characterization and biological assessment of the resulting coatings, the reader is referred to a complementary publication by the authors.</p></div> 2026-09-25T17:46:27Z Dataset Dataset 10.1371/journal.pone.0358441.s003 https://figshare.com/articles/dataset/_p_Data_PLOS_p_/33999864 CC BY 4.0