2026-10-11T18:09:26Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33997768 2026-09-25T17:23:51Z category_4 category_12 category_13 category_14 category_16 category_272 category_39 category_734 category_931 category_61 category_69 portal_5 item_type_3 month_year_09_2026

<p>Test samples.</p> Yi-Ning Huang (2938716) Li-Na Ke (10988956) Bin Yuan (19897) Yongwen Lin (2575333) Biochemistry Cell Biology Genetics Molecular Biology Physiology Environmental Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Information Systems not elsewhere classified Developmental Biology Inorganic Chemistry preliminary theoretical reference g ., ribosome economically important edible core commercial trait cellular homeostasis maintenance biological processes associated nitrogen metabolism ); carbon metabolism ); cellular component formation differentially expressed genes auricularia polytricha </ substance synthesis pathways specific regulatory pathways polytricha </ structure formation specific pattern protein synthesis pathways related pathways involved energy metabolism research gap product quality molecular marker mf ). metabolic activity material support kyoto encyclopedia gene ontology fruiting bodies findings indicate enrichment analyses economic value ear thickness ear thickening directly determines cell proliferation catalytic function assisted breeding <div><p><i>Auricularia polytricha</i> is an economically important edible and medicinal mushroom widely cultivated in China. Ear thickness, as a core commercial trait of this mushroom, directly determines its product quality and economic value. To explore the potential molecular regulatory mechanisms governing ear thickness, we performed comparative transcriptome sequencing on fruiting bodies of the main cultivar ‘Zhang’er 4328’ and its stable thick-ear mutant ‘B2872’ at three key developmental stages: primordium stage (PD), young ear stage (IF), and mature stage (MF). Differentially expressed genes (DEGs) were screened, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. GO enrichment results showed that DEGs were mainly enriched in biological processes associated with metabolic activity, cellular component formation, and catalytic function. KEGG enrichment analysis revealed a distinct stage-specific pattern: DEGs at the PD stage were primarily enriched in energy and substance synthesis pathways (e.g., thiamine metabolism, carbon metabolism); at the IF stage, DEGs were concentrated in pathways related to cell proliferation and structure formation (e.g., amino acid metabolism, nitrogen metabolism); and at the MF stage, DEGs were enriched in pathways involved in protein synthesis and cellular homeostasis maintenance (e.g., ribosome, endoplasmic reticulum protein processing). Our findings indicate that pathways related to energy metabolism, cell proliferation, and protein synthesis provide essential energy and material support for ear thickness in <i>A. polytricha</i>. This exploratory study preliminarily fills the research gap in the molecular mechanism underlying ear thickness variation in <i>A. polytricha</i> and provides a preliminary theoretical reference for molecular marker-assisted breeding of thick-ear varieties.</p></div> 2026-09-25T17:23:34Z Dataset Dataset 10.1371/journal.pone.0358747.t001 https://figshare.com/articles/dataset/_p_Test_samples_p_/33997768 CC BY 4.0