2026-10-12T10:10:59Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33994221 2026-09-25T09:06:26Z category_12 portal_316 item_type_3 month_year_09_2026

Supplementary file 1_Transcription-dependent polyadenylation signal activity contributes to transcriptomic regulation during Forskolin-induced BeWo cell syncytialization.docx Juan Liu (6492) Guolin Ye (17812979) Xin Liu (43569) Haiming Jing (5119184) Shan Gao (46743) Zhongyuan Deng (8995175) Junyu Ning (14555783) Cell Biology polyadenylation polyadenylation signals transcriptional activity placenta trophoblast syncytialization <p>Polyadenylation, a core transcriptional step governed by polyadenylation signals (PAS), functionally interacts with transcription. Nevertheless, how PAS sequences respond to variable transcriptional output remains poorly characterized. Here, we precisely modulated cellular transcription levels by integrating SV40 promoter truncation with a Tet-On dual-luciferase reporter system. Diminished polyadenylation efficiency accompanies lowered promoter activity, and graded transcription assays revealed a tiered PAS processing model: canonical and suboptimal PASs maintain stable 3′-end processing at high transcription levels, whereas non-canonical PASs display compromised processing efficiency with rising transcription. During forskolin(FSK)-induced BeWo cell syncytialization, endogenous trophoblast fusion genes carrying potent canonical PASs were selectively mobilized upon transcriptional activation to enrich differentiation transcripts and sustain orderly syncytiotrophoblast maturation. Together, these findings reveal a hierarchical transcription-polyadenylation axis that associate with placental trophoblast syncytialization. They fill key gaps in mRNA processing and provide a foundation for studying the transcriptional regulatory mechanisms underlying trophoblast differentiation.</p> 2026-09-25T09:06:26Z Dataset Dataset 10.3389/fcell.2026.1958080.s001 https://figshare.com/articles/dataset/Supplementary_file_1_Transcription-dependent_polyadenylation_signal_activity_contributes_to_transcriptomic_regulation_during_Forskolin-induced_BeWo_cell_syncytialization_docx/33994221 CC BY 4.0