2026-10-07T03:28:02Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33993928 2026-09-25T08:18:15Z category_25087 category_24142 item_type_3 month_year_09_2026

Raw targeted TCA cycle metabolomics data from hepatocyte-specific ROMO1-knockout, ROMO1-overexpressing, and control mice Kun Peng (25108294) Metabolomic chemistry Cell metabolism Targeted metabolomics TCA cycle LC-MS/MS Liver Mus musculus <p dir="ltr">Targeted LC-MS/MS profiling of tricarboxylic acid (TCA) cycle metabolites in liver tissues from hepatocyte-specific ROMO1-knockout, ROMO1-overexpressing, and control mice. </p><p><br></p><p dir="ltr">The study comprises 21 male Mus musculus mice (129/SV, 16 weeks old): 4 controls and 5 ROMO1-knockout mice in the hKO cohort, and 7 controls and 5 ROMO1-overexpressing mice in the hOE cohort. Cohorts were analyzed in separate analytical batches using the same method. No pharmacological or dietary treatment was administered.</p><p><br></p><p dir="ltr">Liver tissues were extracted with ice-cold 80% methanol, subjected to four freeze-thaw cycles, and centrifuged at 15,000 x g for 10 min at 4 degrees C. Analysis used a Waters ACQUITY UPLC I-Class system with an Agilent Poroshell 120 SB-Aq column and triple-quadrupole mass spectrometry in negative electrospray ionization, multiple-reaction monitoring mode. Acquisition software: MassLynx v4.2 SCN977.</p><p><br></p><p dir="ltr">Measured metabolites: citrate, alpha-ketoglutarate, succinate, malate, and fumarate. Processed measurements are peak areas (arbitrary units), not absolute concentrations. Raw format: Waters MassLynx RAW directories.</p><p><br></p><p dir="ltr">Sample identifiers: hKO_Control_1 to hKO_Control_4; hKO_1 to hKO_5; hOE_Control_1 to hOE_Control_7; hOE_1 to hOE_5. Prefixes identify the knockout and overexpression cohorts and separate analytical batches.</p><p><br></p><p dir="ltr">Related manuscript: A redox-coupled ROMO1-LONP1-SIRT3 axis controls hepatic lipid catabolism.</p> 2026-09-25T08:18:15Z Dataset Dataset 10.6084/m9.figshare.33993928.v1 https://figshare.com/articles/dataset/Raw_targeted_TCA_cycle_metabolomics_data_from_hepatocyte-specific_ROMO1-knockout_ROMO1-overexpressing_and_control_mice/33993928 CC BY 4.0