2026-10-13T07:40:16Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33993103 2026-09-25T05:32:24Z category_8 portal_316 item_type_3 month_year_09_2026

Data Sheet 1_The diguanylate cyclase CrdA contributes to cell adhesion and gut colonization by switching motility and sessile state of Cronobacter malonaticus.docx Jia Jia (448088) Siqi Li (2283472) Xinlu Yu (13651665) Feiyan Jiao (25107712) Yufei Han (4975952) Jing Zhang (23775) Bingxin Liu (1734601) Meiling Jiang (4720449) Ge Yun (25107715) Dan Liu (108669) Yue Ma (384284) Jiping Yu (20153533) Mingming Song (9903796) Shuwei Yan (15402404) Haifeng Wang (188956) Junlong Zhao (3586115) Yuanyuan Wang (20181) Gukui Chen (6706658) Microbiology bioflim c-di-GMP cell adhesion Cronobacter malonaticus diguanylate cyclases gut colonization motility <p>Cronobacter malonaticus is an emerging foodborne pathogen posing severe threats to vulnerable populations. Despite the established role of the ubiquitous second messenger cyclic diguanylate (c-di-GMP) in bacterial virulence, the specific diguanylate cyclases (DGCs) that orchestrate pathogenicity in C. malonaticus are unknown. Here, we systematically characterized 13 putative DGCs in C. malonaticus and identified CrdA as a pivotal regulator. We demonstrated that CrdA reciprocally inhibits swimming motility and promotes cellulose-dependent biofilm formation by modulating intracellular c-di-GMP levels. Functional and structural analyses revealed that the N-terminal transmembrane domains of CrdA are essential for its dimerization and enzymatic activity. Furthermore, CrdA enhances bacterial adhesion to intestinal epithelial cells and gut colonization in vivo, with its expression being induced upon host cell contact. Mechanistically, CrdA suppresses motility partially through the c-di-GMP effector YcgR and regulates biofilm formation in a BcsA-dependent manner. Collectively, this work elucidates a novel signaling pathway where CrdA integrates environmental cues to orchestrate a virulence-associated lifestyle switch, advancing our understanding of c-di-GMP signaling in Cronobacter pathogenicity and highlighting a potential target for disrupting biofilm-mediated persistence in food systems.</p> 2026-09-25T05:32:24Z Dataset Dataset 10.3389/fmicb.2026.1922582.s001 https://figshare.com/articles/dataset/Data_Sheet_1_The_diguanylate_cyclase_CrdA_contributes_to_cell_adhesion_and_gut_colonization_by_switching_motility_and_sessile_state_of_Cronobacter_malonaticus_docx/33993103 CC BY 4.0