2026-10-12T21:18:08Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33993067 2026-09-25T05:31:29Z category_8 portal_316 item_type_3 month_year_09_2026

Data Sheet 1_Unraveling the microbiological and chemical basis of aroma differences in Great Wall cigars: insights from amplicon sequencing and volatile metabolomics.zip Xiang Dai (46403) Mingxuan Yang (2018164) Yangyang Gao (1602409) Jinshan Lei (18185116) Keyuan Ye (22246219) Ting Zhou (38572) Qi Chen (144168) Yonghao Tan (17734802) Haoyu Yan (17627262) Lanxin Ma (2070763) Yumeng Zhang (3115050) Huina Zhou (1892548) Jun Hu (5721) Xi Hu (773269) Microbiology amplicon sequencing aroma cigar correlation analysis microbial community volatile metabolomics volatile organic compounds <p>To clarify the microbiological and chemical bases underlying aroma differences among six Great Wall cigar types, 16S rRNA/ITS amplicon sequencing and headspace solid-phase microextraction coupled with gas chromatography–mass spectrometry (HS-SPME-GC-MS) were integrated to characterize microbial community composition, volatile organic compound (VOC) profiles, and potential microbe–VOC associations. Microbial diversity and community composition differed among the six cigar types, and linear discriminant analysis effect size (LEfSe) identified 16 potential microbial biomarkers. Volatile metabolomics analysis identified 1,620 VOCs, which were classified into nine chemical classes. The combined results of relative odor activity value (rOAV) analysis and partial least squares discriminant analysis (PLS-DA) revealed 17 VOCs as key aroma compounds associated with aroma differences among the six cigar types. Notably, representative compounds, including β-damascenone (floral, fruity, sweet), dihydro-2-methyl-3(2H)-furanone (roasted, nutty, sweet), δ-cadinene (herbal, woody), and δ-dodecalactone (creamy, fruity), exhibited distinct rOAV distribution patterns across the six cigar types, which may contribute to the characteristic aroma profiles of these cigars. Correlation analysis further revealed that several microbial genera were significantly associated with key aroma compounds. In particular, the bacterial genus Atopostipes and the fungal genus Sampaiozyma displayed significant positive correlations with dihydro-2-methyl-3(2H)-furanone, β-damascenone, and other key aroma compounds. These findings provide useful insights for screening aroma-producing microorganisms and improving cigar quality control.</p> 2026-09-25T05:31:29Z Dataset Dataset 10.3389/fmicb.2026.1952964.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Unraveling_the_microbiological_and_chemical_basis_of_aroma_differences_in_Great_Wall_cigars_insights_from_amplicon_sequencing_and_volatile_metabolomics_zip/33993067 CC BY 4.0