2026-10-13T16:45:43Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33993001 2026-09-25T05:31:19Z category_696 portal_316 item_type_3 month_year_09_2026

Data Sheet 1_A palmitoylation-associated three-gene signature predicts prognosis, immune features, and drug sensitivity in gastric cancer: functional validation of CTHRC1.zip Hongmei Sheng (6801131) Xuejun Li (133209) Chen Zhang (66790) Miaomiao Song (10755624) Juan Xu (225010) Jun Ma (9393) Genetic Immunology drug sensitivity gastric cancer palmitoylation-associated genes prognosis tumor microenvironment Background<p>Gastric cancer (GC) is a heterogeneous malignancy that requires improved molecular tools for prognostic stratification. Protein palmitoylation regulates membrane localization and signaling, but the clinical relevance of palmitoylation-related genes (PRGs) in GC remains unclear.</p>Methods<p>Gene Expression Omnibus, The Cancer Genome Atlas, and GeneCards data were integrated to identify PRGs. Differential expression analysis, weighted gene co-expression network analysis, and three machine-learning algorithms were used to select key genes. Diagnostic and prognostic performance was evaluated in TCGA-STAD, platform-specific GEO cohorts, and an institutional cohort of 80 paired tissues. Immune infiltration, single-cell RNA sequencing, immunotherapy-related indices, and GDSC-based drug sensitivity were analyzed. CTHRC1 was further examined using clinical specimens, cell assays, and xenografts.</p>Results<p>Thirteen differentially expressed PRGs were identified, and SERPINE1, CTHRC1, and EGR1 were selected. The integrated diagnostic model achieved AUCs of 0.935 in TCGA-STAD, 1.000 in GSE54129, and 0.916 in GSE118916, respectively. The three-gene score stratified overall survival in the total, training, and testing TCGA-STAD cohorts and in GSE84437, GSE236522, and the institutional cohort. It remained independently associated with survival in multivariable analysis. High-risk tumors showed increased stromal and immune scores, altered immune-cell composition, higher checkpoint expression, and higher TIDE scores. Single-cell analysis localized SERPINE1 and CTHRC1 mainly to fibroblast-related compartments. CTHRC1 knockdown reduced GC-cell proliferation, clonogenicity, migration, invasion, and xenograft growth while increasing apoptosis.</p>Conclusion<p>The SERPINE1/CTHRC1/EGR1 signature provides prognostic and immune-stromal information in GC, and the experiments support a tumor-promoting role for CTHRC1. The findings define a palmitoylation-associated transcriptional framework but do not establish a palmitoylation-dependent mechanism.</p> 2026-09-25T05:31:19Z Dataset Dataset 10.3389/fimmu.2026.1893438.s001 https://figshare.com/articles/dataset/Data_Sheet_1_A_palmitoylation-associated_three-gene_signature_predicts_prognosis_immune_features_and_drug_sensitivity_in_gastric_cancer_functional_validation_of_CTHRC1_zip/33993001 CC BY 4.0