2026-10-06T20:19:35Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33992617 2026-09-25T04:32:27Z category_303 portal_316 item_type_3 month_year_09_2026

Data Sheet 1_Development of a novel organoid-based two-dimensional system to model the intestinal host–parasite interface in fasciolosis.pdf Judit Serrat (13813371) Mar Siles-Lucas (317121) Toni Aebischer (7834) Christian Klotz (36291) Javier González-Miguel (721327) Clinical Microbiology epithelial barrier function Fasciola hepatica host–parasite interactions organoid-derived epithelial monolayers organoids quantitative proteomics Background<p>The earliest interactions between Fasciola hepatica newly excysted juveniles (FhNEJ) and the intestinal epithelium remain poorly understood, partly due to the limited availability of physiologically relevant in vitro models. Here, we establish a human duodenum organoid-derived epithelial monolayer (ODM) co-culture system to investigate the early host–parasite interface, providing a controlled experimental platform for analysing interactions between FhNEJ and the intestinal epithelium.</p>Methods<p>ODMs representing the human duodenum were co-cultured with either live FhNEJ or a tegument-enriched protein fraction derived from the parasites. The ODM co-culture system was subsequently employed to assess FhNEJ viability and parasite–epithelial interactions together with complementary functional and molecular read-outs. These included measurements of trans-epithelial electrical resistance, fluorescein permeability assays, immunofluorescence analysis of intercellular epithelial junctions, and quantitative proteomic profiling.</p>Results<p>The ODM–FhNEJ co-culture system supported sustained parasite viability and enabled direct visualisation of intimate parasite–epithelial interactions. Functional and structural analyses revealed epithelial barrier dysfunction upon exposure to FhNEJ, whilst quantitative proteomics generated an exploratory molecular dataset identifying candidate proteins associated with epithelial junction organisation, immune signalling, cellular stress, and mechanotransduction.</p>Conclusion<p>Together, these findings establish human intestinal ODMs as a physiologically relevant experimental platform for investigating the earliest stages of F. hepatica infection. By integrating functional, imaging-based and quantitative proteomic analyses within a single experimental framework, this model provides a valuable resource for generating and testing hypotheses regarding host–parasite interactions during early-stage fasciolosis.</p> 2026-09-25T04:32:27Z Dataset Dataset 10.3389/fcimb.2026.1886545 https://figshare.com/articles/dataset/Data_Sheet_1_Development_of_a_novel_organoid-based_two-dimensional_system_to_model_the_intestinal_host_parasite_interface_in_fasciolosis_pdf/33992617 CC BY 4.0