2026-10-12T12:07:07Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33991485 2026-09-25T03:07:55Z category_1 category_4 category_915 category_8 category_12 category_14 category_19 category_272 category_873 category_46 category_734 portal_63 item_type_3 month_year_09_2026

Transcriptomics-Coupled Proteomics Identifies CD36 as a Potential Radiation-Responsive Biomarker in Macrophages Yonglong Yu (3587162) Dongwei Guan (19472086) Yaoying Long (18003796) Pengju Huang (15432228) Lu Wang (45927) Xinlei Liu (128686) Huishan Wang (582391) Jinyi Liu (325830) Xiaolong Tian (6032345) Yimei Feng (6383840) Shijie Yang (409891) Xi Zhang (83736) Biophysics Biochemistry Physical Sciences not elsewhere classified Microbiology Cell Biology Molecular Biology Pharmacology Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Immunology Biological Sciences not elsewhere classified primary mouse bone fatty acid translocase dysfunctional immune microenvironment induced macrophage apoptosis experiments also confirmed scavenger receptor cd36 protein expression levels indicating potential radioprotective cd36 promoted radiation induced injury also known cd36 upregulation vivo </ thereby highlighting therapeutic target therapeutic effect significant increase responsive biomarker radiation injury radiation exposure prominent feature primarily driven potential radiation pharmacological inhibition mitigating radiation mice exposed intrinsic mechanism inflammatory responses inflammatory effects greatly hindered fully understood findings suggest essential role The therapeutic effect of radiotherapy is greatly hindered by radiation injury, primarily driven by a dysfunctional immune microenvironment. Macrophages play an essential role in regulating the radiation response, but their intrinsic mechanism is not fully understood. In this study, we aimed to identify key molecular targets underlying macrophage responses to radiation using omics technologies including transcriptomics and proteomics. We found that the mRNA and protein expression levels of the scavenger receptor CD36 (also known as fatty acid translocase) were significantly upregulated following radiation exposure in both the murine macrophage cell line RAW264.7 and primary mouse bone-marrow-derived macrophages. Additionally, <i>in vivo</i> experiments also confirmed a significant increase in the level of expression of CD36 in macrophages derived from mice exposed to radiation. Pharmacological inhibition of CD36 promoted radiation-induced macrophage apoptosis and inflammatory responses, indicating potential radioprotective and anti-inflammatory effects. These findings suggest that CD36 upregulation is a prominent feature of macrophages in response to radiation exposure, thereby highlighting its potential as a therapeutic target for mitigating radiation-induced injury. 2026-09-24T00:00:00Z Dataset Dataset 10.1021/acs.jproteome.6c00472.s002 https://figshare.com/articles/dataset/Transcriptomics-Coupled_Proteomics_Identifies_CD36_as_a_Potential_Radiation-Responsive_Biomarker_in_Macrophages/33991485 CC BY-NC 4.0