2026-10-11T20:22:42Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33986290 2026-09-24T14:52:43Z category_133 portal_316 item_type_3 month_year_09_2026

Table 1_Temporal dynamics of photosynthetic inhibition, antioxidant defense, and transcriptomic reprogramming in ‘Crimson Seedless’ grapevine under saline-alkali stress.xlsx Chenying Liu (11405201) Hao Wang (39217) Kai Gai (22294237) Yuzhi Zeng (25104511) Songlin Zhang (1580836) Chuan Zhang (187157) Jing Zhang (23775) Tengfei Xu (3155163) Qiang Jin (1449373) Xinyu Wu (187974) Haixia Zhong (2920479) Fuchun Zhang (129371) Zhenfan Yu (7543730) Plant Biology grapevine photosynthetic limitation saline-alkali stress transcriptional regulation VvWRKY47 <p>Soil salinization alkalization is among the most critical abiotic stressors affecting crop productivity yet the molecular physiological mechanisms governing grapevine (Vitis vinifera L.) responses to combined saline-alkali stress remain poorly understood. This study investigated the temporal dynamics of ‘Crimson Seedless’ grapevine plants propagated from cuttings. Integrated morphological transcriptomic physiological data were collected over a 15-day period of progressive saline-alkali stress. The results showed that saline-alkali stress affected photosynthesis in two phases. During the initial days of stress stomatal closure reduced CO₂ uptake as evidenced by parallel drops in Pn Gs Ci. In the later stages non-stomatal limitations dominated. Pn decreased by 88% by day 15 while Ci rose despite low Gs. In addition stress triggered ROS accumulation membrane damage osmotic dehydration. Transcriptomic analysis revealed 3,795 differentially expressed genes (DEGs) at 7 days 4,084 at 15 days. Further analysis revealed that stress upregulated MAPK signaling hormone transduction flavonoid biosynthesis while downregulating photosynthesis-related ribosomal genes. Weighted gene co-expression network analysis (WGCNA) identified VvWRKY47 as a candidate transcription factor. Quantitative real-time PCR (qRT PCR) validation confirmed that VvWRKY47 expression was induced under saline-alkali conditions. Subcellular localization assays showed the nuclear localization of VvWRKY47 phenotypic analysis of transgenic Arabidopsis plants overexpressing VvWRKY47 revealed enhanced growth performance under salt stress compared to empty vector controls.</p> 2026-09-24T14:52:43Z Dataset Dataset 10.3389/fpls.2026.1951854 https://figshare.com/articles/dataset/Table_1_Temporal_dynamics_of_photosynthetic_inhibition_antioxidant_defense_and_transcriptomic_reprogramming_in_Crimson_Seedless_grapevine_under_saline-alkali_stress_xlsx/33986290 CC BY 4.0