2026-10-12T12:07:10Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33983538 2026-09-24T12:25:28Z category_4 category_7 category_8 category_19 category_21 category_272 category_873 category_61 category_133 portal_87 item_type_3 month_year_09_2026

Extraction, structural features, and <i>in vitro</i> bioactivities of polysaccharides from <i>Prunus padus</i> L. berries Yuchun Su (25103970) Tong Liu (107783) Jiayu Wang (336277) Yuming Liu (610387) Song Wang (144220) Yang Sun (132532) Shusheng Wang (389637) Biochemistry Medicine Microbiology Pharmacology Biotechnology Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Developmental Biology Plant Biology Prunus padus L. berries polysaccharide structural characterisation radical scavenging carbohydrase inhibitory activity <p><i>Prunus padus</i> L. berries represent an undervalued temperate medicinal fruit with established ethnobotanical applications, yet comprehensive research on its endogenous polysaccharides remains absent. This study established an ultrasound-assisted hot-water extraction protocol optimised <i>via</i> Box–Behnken response surface methodology (RSM-BBD), achieving a crude polysaccharide yield of 13.46%. Two homogeneous polysaccharide fractions (PPLP2, PPLP3) were isolated using DEAE anion-exchange chromatography. Multi-spectroscopic and chromatographic characterisation revealed distinct backbone architectures: PPLP2 (4.6 kDa) is a neutral β-linked arabinogalactan, while PPLP3(4.4 kDa) is an acidic α-type pectic polysaccharide rich in galacturonic acid, as confirmed by FT-IR spectral analysis. <i>In vitro</i> tests revealed dose-dependent radical-scavenging and anti-carbohydrase effects for both fractions. PPLP3 exhibited superior antioxidant activity (IC₅₀: DPPH<b>^·</b> = 1.25 mg mL⁻¹, ABTS⁺<b>^·</b> = 0.98 mg mL⁻¹, hydroxyl radical = 1.42 mg mL⁻¹). At 1.0 mg mL⁻¹, both polysaccharides inhibited >80% α-amylase and α-glucosidase activity, with respective IC₅₀ below 0.032 and 0.02 mg mL⁻¹. This work constitutes the first integrated structural and biofunctional characterisation of <i>P. padus</i> berry polysaccharides, providing empirical evidence for their development as natural antioxidant and antihyperglycemic nutraceutical ingredients.</p> 2026-09-24T12:25:28Z Dataset Dataset 10.6084/m9.figshare.33983538.v1 https://figshare.com/articles/dataset/Extraction_structural_features_and_i_in_vitro_i_bioactivities_of_polysaccharides_from_i_Prunus_padus_i_L_berries/33983538 CC BY 4.0