2026-10-12T19:58:41Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33979933 2026-09-24T05:33:12Z category_696 portal_316 item_type_3 month_year_09_2026

Table 2_A trivalent gastric cancer antigen conjugated to a diphtheria toxin epitope synergizes with an NKT cell agonist to boost antitumor immunity.xlsx Jing-Jing Du (1973026) Rong-Ling Liu (25102216) Yao-Wei Bai (25102219) Jin-Xuan Su (25102222) Yuan-Mei Zheng (25102225) Zhen-Hong Su (20108646) Shi-Hao Zhou (11994123) Jun Guo (85069) Dong-Yuan Wang (20781941) Ru-Yan Zhang (6443417) Genetic Immunology diphtheria toxoid gastric cancer antigen NKT cell agonist peptide vaccine tumor immunotherapy <p>The gastric cancer-associated antigen MG7-Ag is aberrantly expressed in multiple gastrointestinal malignancies and represents a promising broad-spectrum target for pan-gastrointestinal tumor peptide vaccines; however, its clinical application is hampered by low immunogenicity. To overcome this limitation, we covalently conjugated a diphtheria toxin-derived T-helper epitope to the trivalent antigen MG3, which was constructed by tandem repetition of the monovalent MG1 peptide from the MG7-Ag. A liposomal vaccine was subsequently developed by incorporating DT-MG3 together with the natural killer T (NKT) cell agonist α-galactosylceramide (αGalCer). Immunological evaluation demonstrated that DT-MG3 plus αGalCer elicited significantly higher specific IgG titers than DT-MG3 alone or the unconjugated MG3/αGalCer combination. The vaccine also promoted IFN-γ/IL-4 secretion and boosted CD4⁺/CD8⁺ T cell responses to elevate antigen immunogenicity. Moreover, the high-titer specific antibodies induced by the vaccine also efficiently bound to EAC tumor cells and induced complement-dependent cytotoxicity. In the EAC ascites model, vaccination with DT-MG3/αGalCer substantially reduced malignant ascites accumulation and prolonged the survival of tumor-bearing mice. Collectively, this novel anti-gastric cancer vaccine based on the covalent conjugation of a diphtheria toxin epitope to the trivalent MG3 antigen, provides valuable insights for the rational design of potent and safe fully synthetic peptide-based anti-tumor vaccines.</p> 2026-09-24T05:33:12Z Dataset Dataset 10.3389/fimmu.2026.1903458.s001 https://figshare.com/articles/dataset/Table_2_A_trivalent_gastric_cancer_antigen_conjugated_to_a_diphtheria_toxin_epitope_synergizes_with_an_NKT_cell_agonist_to_boost_antitumor_immunity_xlsx/33979933 CC BY 4.0