2026-10-13T18:31:56Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33979870 2026-09-24T05:33:06Z category_8 portal_316 item_type_3 month_year_09_2026

Supplementary file 1_Application and evaluation of nanopore targeted sequencing in the detection of bacterial and fungal pathogens in leukemia bloodstream infectionsa.doc Qian Yao (1451017) Ling Tang (387186) Daozi Jiang (25102195) Ming Wang (172316) Microbiology application bloodstream infection leukemia nanopore targeted sequencing pathogen identification Objective<p>To evaluate the diagnostic performance and clinical impact of microbial nanopore-targeted sequencing (NTS) technology on pathogenic microorganisms in leukemia patients.</p>Methods<p>In this retrospective observational study, we recruited 206 patients to study the effectiveness of NTS in the identification of infectious pathogens. The detection performance of NTS was compared with that of blood culture (BC).</p>Results<p>The positive rate of NTS was significantly higher than that of BC [62.62% (129/206 cases) vs. 3.88% (8/206 cases)]. Compared with BC, the positive and negative percentage agreement of NTS were 87.50% (95%CI: 47.35–99.68%), (7 of 8 cases) and 38.38% (95%CI: 31.52–45.60%), (76 of 198 cases), respectively. The sensitivity of NTS for diagnosing bloodstream infections was 65.32% (95%CI: 56.21–73.69%), (81/124), which was significantly higher than BC [6.45% (95%CI: 2.84–12.13%), (8/124)], P < 0.001. The specificity of NTS was 41.46% (95%CI: 30.17–53.52%%), (34/82), and that of BC was [100%(95%CI: 95.56–100.00%) (82/82)]. The medication of 32 participants (24.81% [32/129]) was modified by NTS results. Among 206 participants, 29 cases who adjusted their medication based on NTS results showed improved clinical outcomes.</p>Conclusion<p>NTS increases pathogen-detection yield but has low specificity and can be used as an adjunctive diagnostic method for leukemia BSI.</p> 2026-09-24T05:33:06Z Dataset Dataset 10.3389/fmicb.2026.1866353.s003 https://figshare.com/articles/dataset/Supplementary_file_1_Application_and_evaluation_of_nanopore_targeted_sequencing_in_the_detection_of_bacterial_and_fungal_pathogens_in_leukemia_bloodstream_infectionsa_doc/33979870 CC BY 4.0