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oai:figshare.com:article/33979471 2026-09-24T04:33:34Z category_19 portal_316 item_type_3 month_year_09_2026

Data Sheet 1_Hepatoprotective action of Dendrobium officinale polysaccharide against MAFLD: modulation of gut microbiota-metabolite cascade controls TLR4/NF-κB inflammatory response.pdf Yumei Xu (8293980) Yanmei Zhang (122913) Shuqing Li (1430998) Heyan Yao (16942366) Yang Yang (45629) Cuiping Yu (841336) Shunzhen Zhang (169138) Pharmacology Dendrobium officinale polysaccharide intestinal microflora-derived metabolites lipopolysaccharide medicinal and edible homologous plant metabolic dysfunction-associated fatty liver disease TLR4/NF-κB inflammatory pathway Introduction<p>Metabolic dysfunction-associated fatty liver disease (MAFLD) threatens public health worldwide, and Dendrobium officinale polysaccharide (DOP) exhibits promising hepatoprotective potential, yet its regulatory mechanism targeting the gut microbiota-metabolite-TLR4/NF-κB inflammatory axis remains poorly defined.</p>Methods<p>In this study, in-vivo rat and in-vitro HepG2 cell MAFLD models were established. Male Sprague-Dawley (SD) rats received 12-week high-fat diet (HFD) feeding to induce MAFLD; HepG2 cells were exposed to a 1 mmol/L free fatty acid (FFA) mixture (oleic acid/palmitic acid = 2:1) for 24 h to trigger cellular steatosis. Serum, hepatic and cellular biochemical indexes, intestinal permeability factors and inflammatory mediators were detected. Hepatic pathological lesions and lipid accumulation were evaluated via hematoxylin-eosin (HE) and Oil Red O staining. Fecal metagenomics and untargeted metabolomics were applied to profile gut microbes and differential metabolites. RT-qPCR and Western blotting were used to assess tight-junction protein expression and TLR4/NF-κB pathway activation.</p>Results<p>DOP markedly alleviated hepatic lipid accumulation, restored liver function abnormalities and mitigated hepatic pathological damage. DOP reshaped the disturbed gut flora: it reduced lipopolysaccharide (LPS)-enriched Gram-negative bacteria, elevated short-chain fatty acid-producing probiotics, modified intestinal metabolites, repaired the intestinal barrier and prevented intestinal LPS leakage. Both cellular and animal experiments demonstrated that DOP down-regulated TLR4, MyD88 and NF-κB p65 levels, increased IκBα expression and suppressed downstream pro-inflammatory mediators.</p>Discussion<p>Collectively, DOP confers protective effects against MAFLD through remodeling gut microbiota and intestinal metabolites, preserving intestinal-barrier integrity, and inhibiting LPS-provoked hepatic TLR4/NF-κB inflammatory activation.</p> 2026-09-24T04:33:34Z Dataset Dataset 10.3389/fphar.2026.1953606.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Hepatoprotective_action_of_Dendrobium_officinale_polysaccharide_against_MAFLD_modulation_of_gut_microbiota-metabolite_cascade_controls_TLR4_NF-_B_inflammatory_response_pdf/33979471 CC BY 4.0