2026-10-10T01:09:21Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33979204 2026-09-24T04:28:49Z category_303 portal_316 item_type_3 month_year_09_2026

Table 6_Time-dependent transcriptomic response to scrapie exposure in ovine MSC-derived neuron-like cells.csv Adelaida Hernaiz (12093350) José Gil-Guerrero (25101667) Belén Marín (564085) Francisco J. Vázquez (25101670) Juan J. Badiola (12093365) Pilar Zaragoza (122378) Rosa Bolea (220007) Inmaculada Martín-Burriel (122301) Clinical Microbiology early transcriptomic response neuron-like cells ovine mesenchymal stem cells prion RNA-seq scrapie <p>Prion diseases are fatal neurodegenerative disorders in which early molecular responses to prion exposure remain incompletely understood, particularly in naturally susceptible hosts. In the present study, we performed RNA-sequencing analysis of ovine mesenchymal stem cell-derived neuron-like cells exposed to classical scrapie brain homogenate and compared their transcriptomic response with that of cells exposed to normal brain homogenate. Cells were analyzed at 2 and 4 days post-inoculation (dpi). Differential expression analysis identified 76 genes at 2 dpi and 124 genes at 4 dpi, with limited overlap between time points, indicating a dynamic response to scrapie exposure. At 2 dpi, enriched biological processes among upregulated genes were mainly related to signaling- and immune-associated transcriptional responses, whereas downregulated genes were enriched in developmental, morphogenetic and lipid-related processes. At 4 dpi, the transcriptional profile was dominated by broader gene repression and showed reduced functional specificity. RT-qPCR analysis of eight selected genes supported the direction of change detected by RNA-seq in most cases. Overall, these results indicate that scrapie exposure elicits a time-dependent transcriptional response in ovine MSC-derived neuron-like cells and provide an exploratory framework for studying early molecular responses associated with prion exposure in a naturally susceptible species.</p> 2026-09-24T04:28:49Z Dataset Dataset 10.3389/fcimb.2026.1938678.s004 https://figshare.com/articles/dataset/Table_6_Time-dependent_transcriptomic_response_to_scrapie_exposure_in_ovine_MSC-derived_neuron-like_cells_csv/33979204 CC BY 4.0