2026-10-12T13:24:19Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33978587 2026-09-24T03:16:31Z category_1 category_4 category_915 category_12 category_13 category_14 category_21 category_272 category_873 portal_63 item_type_2 month_year_09_2026

Characterization of Ionizable Lipid Membranes for Designing Bioactive Interface of Inorganic Particles Taisei Suzuki (14672184) Keishi Suga (1473559) Ikumi Kato (2320429) Takeru Yamazaki (15411604) Kanako Watanabe (1599223) Tom A. J. Welling (5540219) Hikaru Namigata (17352238) Noriko Yamauchi (2421829) Kumiko Hayashi (13707787) Young-Tae Chang (181448) Satoshi Arai (458400) Daisuke Nagao (1599220) Biophysics Biochemistry Physical Sciences not elsewhere classified Cell Biology Genetics Molecular Biology Biotechnology Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified whereas local heating mcp internalization led elevated local temperatures designing bioactive interface lipid bilayers containing ionizable lipid membranes ionizable cationic lipid containing lipid membranes novel photothermal therapy 150 mt ). liposome characterization revealed 102 slightly decreased lipid membrane properties physicochemical properties membrane fluidity photothermal mcps mcps ). specific roles remain unclear reduced compared potential solution normal conditions foreign materials easily excreted cellular uptake cancer treatment attractive candidates acidic ph 808 nm Magnetic nanoparticles and their composites are attractive candidates for novel photothermal therapy (PTT) agents in cancer treatment. However, the cellular uptake of these artificial particles remains insufficient because foreign materials are easily excreted from cells. Although lipid membrane coating offers a potential solution to this issue, the specific roles of lipid membrane properties, such as membrane fluidity, polarity, and pH responsive surface charge, remain unclear. Herein, we investigated the physicochemical properties of lipid bilayers containing the ionizable cationic lipid, SM-102, and utilized them to functionalize photothermal magnetic composite particles (MCPs). Liposome characterization revealed that the incorporation of SM-102 slightly decreased the membrane fluidity. Coating the photothermal MCPs with SM-102 bilayers successfully imparted pH responsiveness. Consequently, these lipid-coated MCPs (L-MCPs) exhibited enhanced cellular uptake of particles in HeLa cells at acidic pH. Although both uncoated MCPs and L-MCPs were nontoxic under normal conditions, they exhibited cytotoxicity upon simultaneous exposure to NIR (808 nm) light and a direct current magnetic field (150 mT). Local temperature analyses revealed that L-MCP internalization led to elevated local temperatures in the mitochondria, whereas local heating in the lysosomes was reduced compared to pristine MCPs, indicating that coating with SM-102-containing lipid membranes can alter intracellular particle positioning. 2026-09-23T00:00:00Z Dataset Media 10.1021/acs.langmuir.6c04968.s001 https://figshare.com/articles/media/Characterization_of_Ionizable_Lipid_Membranes_for_Designing_Bioactive_Interface_of_Inorganic_Particles/33978587 CC BY-NC 4.0