2026-10-11T20:39:04Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33976786 2026-09-23T17:47:26Z category_4 category_7 category_8 category_13 category_14 category_272 category_46 category_734 category_64 category_132 category_133 category_134 category_135 portal_5 item_type_3 month_year_09_2026

<p>Oligonucleotide primers used in this study.</p> Guiqian Wang (17599253) Pengfei Li (290581) Xinglin He (9899204) Hua Cao (123075) Ahmed H. Ghonaim (12918311) Shengnan Ruan (11411573) Xuexiang Yu (13831981) Jiaru Zhou (19665766) Hongmei Zhu (105489) Mengjia Zhang (2936847) Anan Jongkaewwattana (394525) Guiqing Peng (90055) Qigai He (61080) Wentao Li (1413067) Biochemistry Medicine Microbiology Genetics Molecular Biology Environmental Sciences not elsewhere classified Immunology Biological Sciences not elsewhere classified Cancer Infectious Diseases Plant Biology Virology Computational Biology stranded dna virus global swine industry viral life cycle m5c methylation modifications long multicistronic rnas independent transcription system viral transcription start viral genome annotation asfv transcriptional architecture viral genome transcriptional architecture asfv transcription viral transcripts transcription initiated rna modifications modified rnas asfv ), unmodified transcripts unique host termination sites tail length rna modification predominantly distributed potential functions poses one particularly complex large double identified m6a different lengths accurately identified &# 8221 &# 8220 <div><p>African swine fever virus (ASFV), a large double-stranded DNA virus, poses one of the most significant infectious disease threats to the global swine industry. ASFV transcription is particularly complex because of its unique host-independent transcription system. Using nanopore sequencing technology, we accurately identified the viral transcription start and termination sites. Combining direct RNA sequencing with experimental verification strongly supports the presence of long multicistronic RNAs. By combining this information with the viral genome annotation, we identified six novel transcriptional genes with potential functions. Furthermore, we observed transcriptional “read-through” events, in which transcription initiated at a common start site continues beyond typical termination sites, producing multiple RNA transcripts of different lengths. Additionally, via direct RNA nanopore sequencing, we identified m6A and m5C methylation modifications on viral transcripts, which are predominantly distributed at both termini of the viral genome. Compared to unmodified transcripts, modified RNAs have shorter poly(A) tails, indicating a correlation between RNA modification and poly(A) tail length. Further investigation of ASFV transcriptional architecture, “read-through” events, RNA modifications, and candidate genes may improve our understanding of the viral life cycle and pathogenicity and inform the identification of potential antiviral targets.</p></div> 2026-09-23T17:53:10Z Dataset Dataset 10.1371/journal.ppat.1014617.s008 https://figshare.com/articles/dataset/_p_XXX_p_/33976786 CC BY 4.0