2026-10-08T04:41:08Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33974230 2026-09-25T15:20:41Z category_24625 category_24142 category_24196 category_24649 item_type_3 month_year_09_2026

Lipidomics of human neutrophils during NET formation: PLD1-dependent phosphatidic acid and phosphoinositide remodelling Rahaf Albarghash (25098541) Sergio San-Miguel (25098555) Arturo Zychlinsky (27502) Dhiren Patel (25098424) Innate immunity Cell metabolism Proteomics and metabolomics Medical biochemistry - lipids lipidomics neutrophil extracellular traps phospholipase D1 phosphatidic acid glycerophospholipids <p dir="ltr">Quantitative shotgun lipidomics of primary human neutrophils, underlying Figures 1 and 2 and Supplementary Figures 1 and 4 of Albarghash et al., "Phospholipase D1 activates NAD kinase fuelling neutrophil extracellular trap formation".</p><p dir="ltr">Four files are included; all values are pmol per sample, quantified against lipid class-specific internal standards.</p><p dir="ltr">Supplementary Data 1: PMA time course, lipid class totals. Four conditions (unstimulated; PMA 30, 60 and 180 min) x 3 donors x 3 technical replicates. Underlies Figure 1A and the lipid class composition in Supplementary Figure 1B–E.</p><p dir="ltr">Supplementary Data 2: PMA time course, individual lipid species; the same 36 samples as Supplementary Data 1. Underlies Figure 1B–F.</p><p dir="ltr">Supplementary Data 3: PLD1 inhibitor experiment, individual lipid species. Six conditions (unstimulated; PMA 30 and 180 min; each with vehicle or 1 µM VU0359595) x 3 donors x 2 technical replicates. Underlies Figures 1I–M, 2A–D and Supplementary Figure 4B.</p><p dir="ltr">Supplementary Data 4: PLD1 inhibitor experiment, lipid class totals; the same 36 samples as Supplementary Data 3. Underlies Supplementary Figure 4A.</p><p dir="ltr">Blank cells indicate a lipid not detected in that sample. Each file carries a header block describing its columns, rows and units.</p><p dir="ltr">Neutrophils were isolated from anonymous healthy adult donors with consent and approval from the Charité Hospital ethics committee in Berlin, Germany (EA1/0104/06).</p> 2026-09-25T15:20:41Z Dataset Dataset 10.6084/m9.figshare.33974230.v2 https://figshare.com/articles/dataset/Lipidomics_of_human_neutrophils_during_NET_formation_PLD1-dependent_phosphatidic_acid_and_phosphoinositide_remodelling/33974230 CC BY 4.0