2026-10-08T04:10:41Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33973753 2026-09-23T14:46:30Z category_24361 item_type_3 month_year_09_2026

<b>pGGTOX: a modular plasmid platform for recombination-based genome editing across </b><b><i>Enterobacteriaceae</i></b> Mingju Hao (22496723) Microbial genetics Homologous recombination Genome editing Carbapenem resistance pGGTOX <p dir="ltr">pGGTOX is a modular plasmid system enabling efficient, marker‑free genome editing across multiple Enterobacteriaceae species. It combines rhamnose‑inducible toxin counterselection, sfGFP reporting, Golden Gate cloning, and conjugative transfer to support precise chromosomal and plasmid modifications. Using pGGTOX, we generated targeted deletions (dapA, mrkCD, and a 43‑kb tra cluster) and inserted a 10‑kb CRISPR–Cas9 curing module into diverse hosts. These edits produced expected phenotypes, including reduced biofilm formation, loss of conjugation, and CRISPR‑mediated curing of blaKPC. The dataset provides plasmid maps, sequences, and validation data for genome engineering applications in Enterobacteriaceae.</p> 2026-09-23T14:46:30Z Dataset Dataset 10.6084/m9.figshare.33973753.v1 https://figshare.com/articles/dataset/_b_pGGTOX_a_modular_plasmid_platform_for_recombination-based_genome_editing_across_b_b_i_Enterobacteriaceae_i_b_/33973753 CC BY 4.0