2026-10-13T09:08:36Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33972641 2026-09-23T10:26:47Z category_12 category_13 category_19 category_21 category_873 category_106 portal_63 item_type_2 month_year_09_2026

Dielectrophoretic Motion and Assembly of Janus Liposomes Debneela Paul (25095950) Wei Zhan (526050) Cell Biology Genetics Pharmacology Biotechnology Chemical Sciences not elsewhere classified Science Policy relatively noninvasive technique dependent liposome transformation dependent liposome deformation dependent liposome accumulation 30 mhz ), liposome field alignment ∼ 1 mhz ∼ 1 khz ac frequencies tested results demonstrate dep dependent dep mobility subject microsized liposomes positive mode dep electric field low frequencies intermediate frequencies suitable tool prior investigations mostly generic lipid composition latter category largely unprobed including cells frequently employed domain penetration dielectrophoretic motion dielectric colloids chain formation 10 hz As an electric field-operated and relatively noninvasive technique, dielectrophoresis (DEP) has been frequently employed in the manipulation, assembly and sorting of bioparticles, including cells, organelles and liposomes. Of the latter category, however, prior investigations have been mostly generic, treating liposomes as just another type of dielectric colloids and leaving specific lipid properties, such as their type and phase, largely unprobed. In this work, we subject microsized liposomes in either liquid-ordered or liquid-disordered phase, or their combination, under DEP, and follow their movement and assembly using fluorescence microscopy. Across the range of AC frequencies tested (10 Hz to 30 MHz), we have observed (1) frequency-dependent liposome accumulation, chain formation, size-based sorting, and levitation; (2) two DEP crossover frequency regimes, first from negative to positive mode DEP at ∼1 kHz and then back to negative-mode at ∼1 MHz; (3) lipid phase-dependent liposome transformation and assembly patterns, as manifested by orientation-dependent liposome deformation and domain penetration at low frequencies (10 Hz–300 Hz) and liposome field alignment at intermediate frequencies (10 kHz–300 kHz); and (4) lipid composition-dependent DEP mobility of liposomes. Our results demonstrate DEP as a suitable tool to study lipid phase separation in liposomes. 2026-09-23T00:00:00Z Dataset Media 10.1021/acs.langmuir.6c03808.s004 https://figshare.com/articles/media/Dielectrophoretic_Motion_and_Assembly_of_Janus_Liposomes/33972641 CC BY-NC 4.0