2026-10-12T16:35:43Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33971464 2026-09-23T08:41:28Z category_24367 category_24403 item_type_3 month_year_09_2026

Oxford Nanopore direct RNA sequencing data from MHV-A59 wild-type and nsp15 H262A mutant samples Qingqiu JIANG (25092559) Runsheng Li (9719938) Virology Animal cell and molecular biology MHV-A59 nsp15 H262A Oxford Nanopore direct RNA sequencing RNA004 Warpdemux pod5 Mouse Hepatitis Virus (MHV) <p dir="ltr">This dataset contains Oxford Nanopore direct RNA sequencing data generated from mouse hepatitis virus A59 (MHV-A59) samples, including wild-type and nsp15 H262A mutant virus samples.</p><p dir="ltr">Five RNA samples were multiplexed and sequenced on the same Oxford Nanopore RNA004 flow cell: a mock sample, MHV-wt 1, MHV-wt 3, MHV-mut 1, and MHV-mut 3. Total RNA was extracted using TRIzol reagent, followed by poly(A) RNA enrichment using a VAHTS poly(A) enrichment workflow. Libraries were prepared for Oxford Nanopore direct RNA sequencing using the RNA004 workflow with a custom reverse-transcription-adapter (RTA) barcoding strategy.</p><p dir="ltr">Sequencing was performed on flow cell FAZ51928 from 6–9 March 2026. Reads were demultiplexed using Warpdemux. The deposited dataset contains raw POD5 signal data together with associated sequencing outputs, sample metadata, and documentation describing sample identities and barcode assignments.</p><p dir="ltr">The dataset was generated to support analyses of MHV RNA, including viral transcript profiles and poly(A) tail characteristics. The four virus-containing samples comprise two wild-type and two nsp15 H262A mutant samples. The mock sample was sequenced in the same multiplexed run and is retained as part of the complete sequencing dataset.</p><p dir="ltr">Further information on file organization, sample-to-barcode correspondence, and dataset contents is provided in the accompanying README and sample metadata files.</p> 2026-09-23T08:41:28Z Dataset Dataset 10.6084/m9.figshare.33971464.v1 https://figshare.com/articles/dataset/Oxford_Nanopore_direct_RNA_sequencing_data_from_MHV-A59_wild-type_and_nsp15_H262A_mutant_samples/33971464 CC BY 4.0