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oai:figshare.com:article/33970621 2026-09-23T04:49:08Z category_374 portal_316 item_type_3 month_year_09_2026

Supplementary file 1_Multi-omics profiling and cellular validation reveal distinct lipid networks of krill oil and its predicted mechanisms against insulin resistance.docx Jinxin Lin (2387581) Jinfeng Zhou (124013) Shiyu Luo (502460) Haocheng Liu (6508130) Jiguo Yang (16994720) Clinical and Sports Nutrition 3T3-L1 adipocytes insulin resistance insulin signaling krill oil network pharmacology untargeted lipidomics untargeted metabolomics <p>Insulin resistance is a major contributor to metabolic disorders. We compared krill oil (KO), fish oil (FO), and soybean lecithin (SL) using untargeted metabolomics (UHPLC-HRMS), untargeted lipidomics (UPLC-QTOF-MS), network pharmacology, molecular docking, and a TNF-alpha-induced 3T3-L1 adipocyte model. Metabolomics detected 3,314 annotated features. In the KO versus FO comparison, 378 features were up-regulated and 593 were down-regulated in positive-ion mode, whereas 365 were up-regulated and 925 were down-regulated in negative-ion mode. Lipidomics detected 4,667 lipid-associated features and revealed distinct product-specific patterns of phospholipids, free fatty acids, glycerolipids, and sphingolipids. Network pharmacology identified 394 intersecting candidate targets, with AKT1, IL6, PPARG, and SRC prioritized as high-centrality nodes. Molecular docking was used to prioritize predicted component-target interactions. In mature 3T3-L1 adipocytes, TNF-alpha reduced insulin-stimulated 2-NBDG uptake. KO, FO, and SL increased glucose uptake relative to the TNF-alpha plus insulin group, with KO showing the largest response among the tested oils. KO also increased the p-AKT/AKT ratio and altered the p-IRS1/IRS1 ratio. These findings support compositional differences among the tested oils and cell-level modulation of insulin signaling by KO. They do not establish in vivo efficacy, direct target engagement, or an effect attributable solely to phospholipid form because EPA/DHA exposure was not dose-matched.</p> 2026-09-23T04:49:08Z Dataset Dataset 10.3389/fnut.2026.1925480 https://figshare.com/articles/dataset/Supplementary_file_1_Multi-omics_profiling_and_cellular_validation_reveal_distinct_lipid_networks_of_krill_oil_and_its_predicted_mechanisms_against_insulin_resistance_docx/33970621 CC BY 4.0