2026-10-10T01:39:33Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33970375 2026-09-23T04:42:37Z category_1325 portal_316 item_type_3 month_year_09_2026

Data Sheet 1_Epidemiological insights into virulence and antimicrobial resistance of Escherichia coli across dairy farms in Nagpur district.pdf Tippa Padmaja (25093879) Gayatri Jayaraj (25093882) Yashwarya Dharne (25093885) Ashwini Tulshiram Nerikar (25093888) Shreya Bhojane (25093891) S. P. Chaudhari (25093894) S. V. Shinde (4263466) W. A. Khan (25093897) N. V. Kurkure (25093900) M. P. Kaore (25093903) Rajpal Singh Kashyap (18692514) M. S. Patil (25093918) Veterinary Anaesthesiology and Intensive Care Escherichia coli MAR index multidrug resistance One Health organized dairy farms seasonal variation virulence <p>Antimicrobial-resistant Escherichia coli (E. coli) circulating across the human-animal-environmental interface is a major one-health concern. This study (April 2025–January 2026) investigated the occurrence, virulence, and antimicrobial resistance (AMR) patterns of E. coli across 558 samples comprising animal (n = 444), environmental (n = 80), and human (n = 34) sources collected from five organized dairy farms representing urban (n = 1), peri-urban (n = 2), and rural (n = 2) settings under a One Health framework. Samples were cultured for E. coli and E. coli O157, screened for Shiga-toxin genes (stx1, stx2) by polymerase chain reaction (PCR), and subjected to antimicrobial susceptibility testing and molecular detection of resistant genes. Overall, E. coli was detected in 70.06% of the samples, with the highest occurrence in environmental samples (78.75%), followed by animal (70.94%) and human (47.05%) samples, indicating a clear environmental predominance. In contrast, E. coli O157 was not detected in any of the samples. Virulence profiling revealed the presence of Shiga-toxin encoding genes stx1 (10.1%) and stx2 (13.7%), predominantly among animal isolates, with higher occurrence during monsoon and winter seasons. Antimicrobial susceptibility testing revealed high susceptibility to colistin (100%), tigecycline (97.2%), and carbapenems (>91%), while moderate resistance was observed against tetracycline (25.1%) and selected cephalosporins. Multidrug resistance (MDR) was identified in 18.02% of isolates, with an overall MAR index of 0.068. Molecular analysis showed a predominance of AmpC (33.25%), followed by tetA (20.05%), blaTEM (15.48%), blaCTX-M (12.18%), tetB (9.39%), and blaNDM (2.28%) genes, with notable seasonal variation. These findings indicate that the livestock ecosystem acts as a reservoir for resistant and potentially virulent E. coli, underscoring the need for integrated One Health AMR surveillance and targeted mitigation strategies.</p> 2026-09-23T04:42:37Z Dataset Dataset 10.3389/fvets.2026.1915714.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Epidemiological_insights_into_virulence_and_antimicrobial_resistance_of_Escherichia_coli_across_dairy_farms_in_Nagpur_district_pdf/33970375 CC BY 4.0