2026-10-12T13:47:05Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33970315 2026-09-23T04:28:12Z category_19 portal_316 item_type_3 month_year_09_2026

Data Sheet 1_Protective effects of Lactobacillus rhamnosus GG and 5-methoxyindole-3-acetic acid against diquat-induced acute liver injury involve activation of the Nrf2/HO-1 signaling pathway.pdf Yu Wang (12152) Wenbin Gao (825538) Yonghong Wang (61187) Hongxuan Liu (14408982) Jiahao Wei (8682204) Xiaohong Zhang (32960) Jing Li (10611) Pharmacology 5-methoxyindole-3-acetic acid acute liver injury diquat Lactobacillus rhamnosus GG Nrf2/HO-1 signaling pathway oxidative stress Background<p>Diquat (DQ), a widely used bipyridyl herbicide, induces acute liver injury primarily through oxidative stress, and effective targeted therapies remain lacking. Lactobacillus rhamnosus GG (LGG) exhibits antioxidant potential, but its role in DQ-induced liver injury has not been fully clarified. This study investigated the protective effects of LGG and its associated metabolite 5-methoxyindole-3-acetic acid (5-MIAA) and explored the underlying mechanisms.</p>Methods<p>A DQ-induced acute liver injury model was established in C57BL/6 mice with LGG pretreatment and Nrf2 inhibitor (ML385) intervention. Liver function, histopathology, oxidative stress markers, and Nrf2/HO-1 expression were assessed. In vitro, DQ-treated HepG2 cells were exposed to varying concentrations of 5-MIAA, and cell viability and pathway-related molecules were analyzed using CCK-8 and Western blot.</p>Results<p>DQ exposure markedly elevated ALT and AST levels, aggravated hepatic necrosis and inflammation, increased ROS and MDA levels, decreased SOD and GSH-Px activities, and suppressed Nrf2 and HO-1 expression. LGG pretreatment significantly alleviated liver injury and oxidative stress while restoring Nrf2/HO-1 expression. ML385 partially attenuated the protective effects of LGG. In vitro, 5-MIAA dose-dependently improved viability of DQ-injured HepG2 cells and upregulated Nrf2 and HO-1 expression.</p>Conclusion<p>LGG mitigates DQ-induced acute liver injury by activating the Nrf2/HO-1 pathway and reducing oxidative stress, while 5-MIAA may represent a potential bioactive compound with similar hepatoprotective effects. These findings provide mechanistic support for probiotic-based strategies in toxicant-induced liver injury.</p> 2026-09-23T04:28:12Z Dataset Dataset 10.3389/fphar.2026.1904443.s002 https://figshare.com/articles/dataset/Data_Sheet_1_Protective_effects_of_Lactobacillus_rhamnosus_GG_and_5-methoxyindole-3-acetic_acid_against_diquat-induced_acute_liver_injury_involve_activation_of_the_Nrf2_HO-1_signaling_pathway_pdf/33970315 CC BY 4.0