2026-10-09T22:25:52Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33966866 2026-09-22T17:25:01Z category_1 category_4 category_7 category_8 category_12 category_13 category_14 category_16 category_45 category_61 category_128 portal_5 item_type_3 month_year_09_2026

<p>Raw Data.</p> Xinyan Gan (25091126) Qiwen Li (772694) Qiuchan Xiong (9438477) Denghao Huang (25091129) Zizheng Liu (751836) Qi Yin (279795) Shuang Jiang (1443706) Takuma Matsubara (13524187) Shoichiro Kokabu (381370) Wei Yan (4327) Quan Yuan (83568) Biophysics Biochemistry Medicine Microbiology Cell Biology Genetics Molecular Biology Physiology Sociology Developmental Biology Hematology hair follicle morphogenesis hair follicle cycle establishing cell identity ensure structural integrity diverse functional impacts essential regulatory layers div >< p accelerates hair regeneration rna modifications constitute mettl1 deficiency destabilizes findings establish mettl1 dsg4 signaling axis disrupted keratinocyte adhesion key transcriptional circuit 7 </ sup mediated internal mrna dsg4 axis n </ mettl1 </ key enzyme impaired regeneration essential role >< sup transcriptional regulation transcriptional control well recognized turn downregulates transcription factors specific knock recently emerged protein synthesis pivotal role pivotal layer keratinocytes leads critical regulator conditional knockout cells control <div><p>Rapid hair follicle cycling requires precise fate commitment and differentiation of hair follicle stem cells. While the essential role of transcription factors in establishing cell identity is well recognized, how cells control its protein synthesis to achieve tissue specificity remains unknown. RNA modifications constitute a pivotal layer of post-transcriptional regulation for protein synthesis. Among them, the <i>N</i>⁷-methylguanosine (m⁷G) modification has recently emerged as a critical regulator with diverse functional impacts. Here, we reveal a pivotal role of METTL1, the key enzyme for RNA m⁷G modification, in hair follicle cycle. Conditional knockout of <i>Mettl1</i> in keratinocytes leads to severe hair follicle developmental dysplasia, impaired regeneration, and disrupted keratinocyte adhesion. Complementarily, keratinocyte-specific knock-in of <i>Mettl1</i> accelerates hair regeneration. Mechanistically, beyond its effects on tRNAs, METTL1 deficiency destabilizes <i>HOXC13</i> mRNA through internal m⁷G modification, which in turn downregulates the HOXC13/FOXN1/DSG4 signaling axis. Our findings establish METTL1-mediated internal mRNA m⁷G methylation as one of the essential regulatory layers of RNA modification in hair follicle morphogenesis and cycling, operating through the precise post-transcriptional control of a key transcriptional circuit to ensure structural integrity and timely regeneration.</p></div> 2026-09-22T17:24:42Z Dataset Dataset 10.1371/journal.pgen.1012307.s012 https://figshare.com/articles/dataset/_p_Raw_Data_p_/33966866 CC BY 4.0