2026-10-07T14:21:20Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33966160 2026-09-24T22:06:07Z category_24178 category_24148 category_24169 category_24403 category_28885 portal_295 item_type_3 month_year_09_2026

Manual nuclei segmentations in near-isotropic, aligned volume electron microscopy (eFIB-SEM) of Mosquito stylet (jrc_mosquito-stylet-6) CellMap Project Team (14322654) Alyson Petruncio (9536023) Biochemistry and cell biology not elsewhere classified Cellular interactions (incl. adhesion, matrix, cell wall) Structural biology (incl. macromolecular modelling) Animal cell and molecular biology Image processing FIB-SEM Focused Ion Bean-Scanning Electron Microscopy Electron Microscopy Segmentations <p dir="ltr"><b>Annotation description</b>: Dense segmentations of nuclei in jrc_mosquito-stylet-6 using Amira-Avizo (Thermo Fisher Scientific) and "Using Amira to manually segment organelles in vEM for machine learning V.3" protocol.</p><p dir="ltr"><b>Annotation protocol</b>: Using Amira to manually segment organelles in vEM for machine learning V.3 (<a href="http://dx.doi.org/10.17504/protocols.io.bp2l61rb5vqe/v3" target="_blank">http://dx.doi.org/10.17504/protocols.io.bp2l61rb5vqe/v3</a>)</p><p dir="ltr"><b>Software</b>: Amira 3D 2021.1, Classic Segmentation Workroom</p><p dir="ltr"><b>Label voxel size (nm)</b>: 32 x 32 x 32 (x, y, z)</p><p dir="ltr"><b>Reconstructed data dimensions (µm)</b>: 50.88 x 44.22 x 295.01 (x, y, z)</p><p dir="ltr"><b>Classes annotated</b>: Nucleus</p><p dir="ltr"><b>Source dataset (EM) ID</b>: jrc_mosquito-stylet-6</p><p dir="ltr"><b>Source dataset (EM) voxel size (nm)</b>: 8 x 8 x 8 (x, y, z)</p><p dir="ltr"><b>Source dataset (EM) data dimensions (µm)</b>: 50.88 x 44.23 x 295.02 (x, y, z)</p><p dir="ltr"><b>Source dataset (EM) DOI</b>: <a href="https://doi.org/10.25378/janelia.33050051" target="_blank">https://doi.org/10.25378/janelia.33050051</a></p><p dir="ltr"><b>Dataset URL</b>: <a href="" target="_blank">s3://janelia-cosem-datasets/jrc_mosquito-stylet-6/jrc_mosquito-stylet-6.zarr/labels/inference/segmentations/</a></p><p dir="ltr"><b>Visualization website</b>: <a href="https://openorganelle.janelia.org/datasets/jrc_mosquito-stylet-6" target="_blank">https://openorganelle.janelia.org/datasets/jrc_mosquito-stylet-6</a></p> 2026-09-24T22:06:07Z Dataset Dataset 10.25378/janelia.33966160.v3 https://figshare.com/articles/dataset/Refined_automatic_nuclei_segmentations_in_near-isotropic_aligned_volume_electron_microscopy_eFIB-SEM_of_Mosquito_stylet_jrc_mosquito-stylet-6_/33966160 CC BY 4.0