2026-10-12T07:30:01Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33965236 2026-09-22T13:47:18Z category_16 portal_316 item_type_3 month_year_09_2026

Table 1_Kaempferol ameliorates gestational diabetes mellitus by regulating STAT3-associated AIM2 inflammasome activation and ferroptotic injury in trophoblasts.docx Ronghui Liu (1638166) Qiqi Liu (381557) Lanjie Yi (25090279) Physiology AIM2 inflammasome ferroptosis gestational diabetes mellitus kaempferol STAT1/IRF1 STAT3 Introduction<p>To investigate the protective effects of kaempferol (KPF) against gestational diabetes mellitus (GDM) and elucidate its underlying molecular mechanisms.</p>Methods<p>A GDM rat model was established to assess metabolic abnormalities, inflammation, oxidative stress, and placental injury following KPF treatment. In vitro, high glucose (HG)-stimulated HTR-8/SVneo trophoblast cells were used to investigate the protective mechanisms of KPF. Mitochondrial dysfunction, lipid peroxidation, ferroptosis-related alterations, and AIM2 inflammasome activation were evaluated. STAT3 knockdown and AIM2 overexpression were further performed to determine their roles in HG-induced trophoblast injury.</p>Results<p>KPF significantly improved glucose and lipid metabolism, reduced inflammatory cytokine levels, alleviated placental injury, and decreased oxidative stress in GDM rats. In HG-stimulated trophoblasts, KPF attenuated mitochondrial dysfunction, lipid peroxidation, and ferroptosis-related alterations by restoring GPX4 and SLC7A11 expression and inhibiting AIM2 inflammasome activation. Mechanistically, KPF suppressed STAT3 activation, while STAT3 knockdown reduced STAT1/IRF1 activation and AIM2 expression under hyperglycemic conditions. AIM2 overexpression aggravated ferroptosis-related changes and inflammatory responses.</p>Conclusion<p>KPF alleviates GDM-associated placental dysfunction by modulating STAT3-associated signaling and AIM2 inflammasome activation, thereby reducing ferroptosis-related injury and inflammatory activation in trophoblasts. These findings provide new insights into the mechanisms of GDM and support KPF as a potential therapeutic candidate.</p> 2026-09-22T13:47:18Z Dataset Dataset 10.3389/fphys.2026.1857741.s001 https://figshare.com/articles/dataset/Table_1_Kaempferol_ameliorates_gestational_diabetes_mellitus_by_regulating_STAT3-associated_AIM2_inflammasome_activation_and_ferroptotic_injury_in_trophoblasts_docx/33965236 CC BY 4.0