2026-10-14T04:56:36Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33965056 2026-09-22T13:08:33Z category_696 portal_316 item_type_3 month_year_09_2026

Table 1_A recombinant Newcastle disease virus-vectored multi-epitope vaccine confers protection against QX-like infectious bronchitis virus in chickens.docx Fei Wang (19534) Jianan Xie (19256932) Guoyuan Wen (3474530) Xusheng Qiu (453653) Yang Qu (681035) Yingjie Sun (849052) Cuiping Song (849051) Ying Liao (335547) Chan Ding (317445) Shunlin Hu (849053) Lei Tan (211429) Genetic Immunology immune-protection infectious bronchitis virus multi-epitope vaccine Newcastle disease virus QX-like genotype vaccine vector <p>Infectious bronchitis virus (IBV) QX-like (GI-19) genotype dominates global poultry flocks, yet Mass-type vaccines fail to confer cross-protection. We developed a recombinant Newcastle disease virus (NDV) LaSota vector expressing conserved IBV S1 B-cell hotspots paired with S1-derived BF2-restricted CTL epitopes (rNDV-IBV-T/B). Specific-pathogen-free chickens received single or booster ocular-nasal immunization (10⁶ EID₅₀) followed by QX-like challenge. Booster immunization increased the frequency of splenic CD3⁺CD8⁺ T cells (41.3% vs. 31.9% in the sham-vaccinated challenge control; Tukey-adjusted p<0.001) and elicited neutralizing antibodies (peak log₂ GMT, 5.80). Vaccinated groups showed markedly reduced clinical scores, preserved tracheal ciliary activity at all assessed post-challenge time points, and mitigated histopathological lesions. Vaccination reduced oropharyngeal and cloacal viral RNA shedding at multiple post-challenge time points, with the booster regimen producing a more consistent reduction in cloacal viral RNA. Protection was accompanied by an increased frequency of splenic CD3⁺CD8⁺ T cells, particularly after booster immunization, suggesting a role for cellular immunity alongside neutralizing antibodies. The NDV-LaSota backbone functions as a modular platform for rapid vaccine redesign against emerging coronavirus variants.</p> 2026-09-22T13:08:33Z Dataset Dataset 10.3389/fimmu.2026.1949497.s001 https://figshare.com/articles/dataset/Table_1_A_recombinant_Newcastle_disease_virus-vectored_multi-epitope_vaccine_confers_protection_against_QX-like_infectious_bronchitis_virus_in_chickens_docx/33965056 CC BY 4.0