2026-10-14T08:58:30Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33963212 2026-09-22T08:40:02Z category_146 category_7 category_29 category_46 category_61 category_128 category_132 category_135 portal_87 item_type_3 month_year_09_2026

Developmental differences in platelet P-selectin expression modulate neonatal monocyte inflammatory responses Erin Soule-Albridge (25088618) Emily Flahardy (25088621) Henry A. Feldman (7545905) Natalie Kane (8337708) Kimberley Banfield (25088624) Amalia El Nems (25088627) Jesselin Romero Escobar (17904597) Martha Sola-Visner (5571368) Patricia Davenport (11082074) Space Science Medicine Geology Immunology Developmental Biology Hematology Infectious Diseases Computational Biology Monocyte neonate transfusion <p>Neonatal and adult platelets are functionally different, with neonatal platelets being comparatively hypo-reactive to multiple platelet agonists. When transfused, neonates receive platelets from adult donors, resulting in a cellular developmental mismatch. Platelets are key regulators of the monocyte inflammatory response, yet how the developmental stage of the platelet affects this response is unknown. In this study, we modeled this developmental mismatch <i>in vitro</i> to investigate the neonatal (cord blood) monocyte inflammatory response and migratory phenotype after exposure to either neonatal (cord blood) or adult platelets. Adult platelets expressed more P-selectin on the surface and released more beta-2-microglobulin (B2M) after activation compared to neonatal platelets. While exposure to platelets of either developmental stage increased the release of IL-8 and MCP-1 from neonatal monocytes to a similar degree, exposure to adult platelets induced significantly greater monocyte CCR2 and CCR5 surface expression, consistent with a pro-migratory monocyte phenotype. Blocking P-selectin/PSGL-1 interactions had no effect on platelet induced monocyte cytokine release but completely abrogated monocyte CCR2 and CCR5 surface upregulation. These findings suggest that the monocyte pro-migratory phenotype is regulated by P-selectin exposure, which is influenced by the platelet developmental stage, while monocyte cytokine release is independent of platelet P-selectin expression or developmental stage.</p> <p><b>What is the context?</b></p> <p></p><p>Premature neonates in the neonatal intensive care unit (NICU) often receive platelet transfusions to prevent bleeding, but use of higher transfusion thresholds has been linked to increased death, bleeding, lung disease, and developmental delays.</p><p>This may be due to a “mismatch” between the neonate’s platelets and the transfused platelets from adult donors. Compared to neonatal platelets, adult platelets are more active and potentially more inflammatory.</p><p>Platelets help regulate inflammation through interactions with immune cells like monocytes, but how exposure to neonatal vs. adult platelets affects the monocyte inflammatory response is unknown.</p><p></p> <p>Premature neonates in the neonatal intensive care unit (NICU) often receive platelet transfusions to prevent bleeding, but use of higher transfusion thresholds has been linked to increased death, bleeding, lung disease, and developmental delays.</p> <p>This may be due to a “mismatch” between the neonate’s platelets and the transfused platelets from adult donors. Compared to neonatal platelets, adult platelets are more active and potentially more inflammatory.</p> <p>Platelets help regulate inflammation through interactions with immune cells like monocytes, but how exposure to neonatal vs. adult platelets affects the monocyte inflammatory response is unknown.</p> <p><b>What is new?</b></p> <p></p><p>In this study, we found that monocytes isolated from neonates responded differently after exposure to adult vs. neonatal platelets.</p><p>Exposure to adult platelets increased CCR2 and CCR5 on monocytes, priming them to migrate from blood into tissue – an effect driven by platelet P-selectin binding to the monocyte receptor PSGL-1.</p><p></p> <p>In this study, we found that monocytes isolated from neonates responded differently after exposure to adult vs. neonatal platelets.</p> <p>Exposure to adult platelets increased CCR2 and CCR5 on monocytes, priming them to migrate from blood into tissue – an effect driven by platelet P-selectin binding to the monocyte receptor PSGL-1.</p> <p><b>What is the Impact?</b></p> <p></p><p>These findings show the need to better understand how platelet immune function changes with development, so transfusion products can be better matched to a patient’s age – reducing harmful inflammation and improving outcomes.</p><p></p> <p>These findings show the need to better understand how platelet immune function changes with development, so transfusion products can be better matched to a patient’s age – reducing harmful inflammation and improving outcomes.</p> 2026-09-22T08:40:02Z Dataset Dataset 10.6084/m9.figshare.33963212.v1 https://figshare.com/articles/dataset/Developmental_differences_in_platelet_P-selectin_expression_modulate_neonatal_monocyte_inflammatory_responses/33963212 CC BY 4.0