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oai:figshare.com:article/33962305 2026-09-22T05:40:55Z category_374 portal_316 item_type_3 month_year_09_2026

Supplementary file 1_Structural characterization and in vitro myocardial injury protection potential of one acidic polysaccharide from blueberry (Vaccinium spp.) pomace.docx Beibei Zhang (509356) Qiwen Zhang (490414) Ning Huang (623370) Mengmeng Jia (1625968) Han Xing (227847) Yongjie Yang (3187218) Dinglei Xue (25087996) Jing Yang (13969) Zifei Qin (5643410) Clinical and Sports Nutrition acidic pectin polysaccharide blueberry pomace myocardial injury protection potential PI3K/AKT/mTOR and MAPK pathways structural characterization Introduction<p>Blueberry pomace is the by–product of blueberry (Vaccinium spp.) fruits. This study aimed to prepare blueberry pomace polysaccharide (BBPP) and explore its myocardial injury protection potential.</p>Methods<p>An acidic polysaccharide (BBPP80–1a) was isolated and purified from active BBPP80-1 fraction. Its structure was characterized based on some approaches. Its myocardial injury protection potential was performed in hypoxia/reoxygenation (H/R)-induced H9c2 cells injury model.</p>Results<p>The molecular weight of BBPP80–1a was 164.91 kDa. The ratio of galacturonic acid (GalA) was 50.92% in methylation results. Further, nuclear magnetic resonance data suggested repetitive → 4)-α-D-GalpA-(1 → and → 4)-α-D-GalpA-6-OMe-(1 → residues occupied the homogalacturonan region of backbone. It had a highly porous, reticular, and heterogeneous microstructures. Moreover, BBPP80–1a can obviously protect H9c2 cells from H/R injury via phosphoinositide 3-kinase / protein kinase B / mammalian target of rapamycin (PI3K/AKT/mTOR) and c-Jun N-terminal kinase / mitogen-activated protein kinase (JNK/MAPK) pathways.</p>Discussion<p>BBPP80–1a is a pectic polysaccharide. It exhibited the potential on cardioprotective effect through the activation of PI3K/AKT/mTOR survival pathway and the regulation of JNK/MAPK pathways.</p> 2026-09-22T05:40:55Z Dataset Dataset 10.3389/fnut.2026.1920321.s001 https://figshare.com/articles/dataset/Supplementary_file_1_Structural_characterization_and_in_vitro_myocardial_injury_protection_potential_of_one_acidic_polysaccharide_from_blueberry_Vaccinium_spp_pomace_docx/33962305 CC BY 4.0