2026-10-14T04:46:41Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33959875 2026-09-21T20:36:03Z category_23830 category_23824 item_type_3 month_year_09_2026

<b>Allelic replacement of the hexose transporter gene Lr67 by prime editing mimics natural durable resistance to leaf rust in hexaploid wheat</b> Rafael Venado (25085437) Joshua Waites (20778941) Easter D. Syombua (20778947) Anindya Bandyopadhyay (3280794) Genetically modified field crops and pasture Agricultural molecular engineering of nucleic acids and proteins wheat rust disease gene editing constructs <p dir="ltr">This study describes the development and application of a wheat-compatible prime-editing system to introduce the Lr67-associated TaSTP13(G144R) resistance allele into the A, B, and D TaSTP13 homoeologs of hexaploid wheat. Edited lines were generated and characterized for editing efficiency, followed by evaluation of leaf rust resistance using disease severity, fungal biomass quantification, and defense-related gene expression. The results demonstrate the potential of prime editing to directly introduce resistance-associated alleles into wheat.</p> 2026-09-21T20:36:03Z Dataset Dataset 10.6084/m9.figshare.33959875.v1 https://figshare.com/articles/dataset/_b_Allelic_replacement_of_the_hexose_transporter_gene_Lr67_by_prime_editing_mimics_natural_durable_resistance_to_leaf_rust_in_hexaploid_wheat_b_/33959875 CC BY 4.0