2026-10-11T19:59:39Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33955896 2026-09-21T17:26:08Z category_1 category_12 category_13 category_14 category_24 category_39 category_734 category_62 portal_5 item_type_3 month_year_09_2026

<p>Supplementary tables.</p> Alex Junior Aparecido Silvestrini (25084461) Larissa Ramsay (713662) Eric Bishop von Wettberg (25084464) Kirstin E. Bett (12895100) Biophysics Cell Biology Genetics Molecular Biology Evolutionary Biology Ecology Biological Sciences not elsewhere classified Marine Biology level similarity among div >< p rearrangements pose challenges six wild relatives confirm genome assemblies lens culinaris </ green oligo probes wild relatives rearrangements detected genome assemblies lens </ situ </ significant role robust karyotype results attest proper assignment probes resulted precise representation precise karyotype karyotype evolution important cool higher sequence distinct pattern developed cross affect introgression accurate karyotype <div><p>Chromosome structural rearrangements play a significant role in karyotype evolution and speciation. These rearrangements pose challenges for precise karyotyping and the assembly of a genus pan-genome for crops and their wild relatives. <i>Lens culinaris</i>, an important cool-season legume primarily grown in India and Canada, is a cultivated species with six wild relatives. All seven <i>Lens</i> species exhibit distinct chromosomal arrangements, which affect introgression and complicate the development of an accurate karyotype for the genus. Using gene synteny analysis between the cultivated <i>Lens</i> species and six wild relatives, we developed cross-species oligo-FISH (Fluorescent <i>in situ</i> hybridization) probes to <i>in situ</i> confirm genome assemblies and synteny. Chromosome spreads were used for oligo-FISH experiments, in which the DNA was denatured and a set of red and green oligo probes was hybridized to the metaphase chromosomes. The combination of both oligo sets/probes resulted in a distinct pattern for each <i>Lens</i> spp. chromosome, allowing the inference of a more robust karyotype for six <i>Lens</i> species. The karyotyping of <i>Lens</i> spp. confirmed the proper assignment of chromosomes in the genome assemblies and validated the rearrangements detected in the synteny analysis. The results attest to a higher sequence-level similarity among the closest related species despite the occurrence of several chromosomal structural changes among them. Oligo-FISH probes can be used in conjunction with plant genome assembly projects, supporting the delivery of a precise representation of the physical chromosomes of a species.</p></div> 2026-09-21T17:26:04Z Dataset Dataset 10.1371/journal.pone.0354141.s002 https://figshare.com/articles/dataset/_p_Supplementary_tables_p_/33955896 CC BY 4.0