2026-10-11T21:27:18Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33954647 2026-09-21T14:04:12Z category_4 category_7 category_12 category_13 category_14 category_24 category_272 category_873 category_734 portal_63 item_type_3 month_year_09_2026

A Pangenome Analysis of the RPD3/HDA1-Type Histone Deacetylase (HDAC) Family in Musaceae Unveils MaHDAC3 as an Epigenetic Repressor of Fruit Ripening Zhiwei Lu (129184) Shiyu Lu (1474159) Xiaowan Hou (752134) Qiang Chen (114038) Weijun Xiao (12088900) Zhiwei Jia (434311) Yajie Duan (3400061) Yulin Hu (3400064) Zhencai Pang (8385768) Dequan Sun (1602352) Jianghui Xie (3400067) Huigang Hu (3400070) Biochemistry Medicine Cell Biology Genetics Molecular Biology Evolutionary Biology Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified ii remained constrained distinct subcellular landscape boosting glycolytic flux 20 representatives unveiling type histone deacetylase evolutionary analyses revealed critical epigenetic modulators musaceae unveils mahdac3 type members evolutionary plasticity defines mahdac3 epigenetic repressor epigenetic brake study highlights shaped primarily ros accumulation ripening inhibitor promising target pangenomic investigation pangenome analysis limiting genes genome duplications fruit ripening evolutionarily dynamic dichotomy within derepressing rate carbon metabolism accelerated sugar Histone deacetylases (HDACs) are critical epigenetic modulators of fruit ripening. In plants, the RPD3/HDA1 subfamily is the most evolutionarily dynamic and functionally diverse group within the HDAC superfamily. To decipher how the RPD3/HDA1 subfamily coordinates fruit ripening, we conducted a pangenomic investigation of these members across 18 Musaceae genomes. A total of 199 RPD3/HDA1-type members were identified, with 20 representatives unveiling a distinct subcellular landscape. Evolutionary analyses revealed a dichotomy within the subfamily: Class-I expanded via multiple duplications, while Class-II remained constrained, shaped primarily by dispersed and whole-genome duplications. Transcriptional and functional screening identified MaHDAC3 as a ripening inhibitor, and its silencing triggered premature degreening and accelerated sugar/ROS accumulation. MaHDAC3 deficiency elevated global histone acetylation, derepressing rate-limiting genes and boosting glycolytic flux. Collectively, our study highlights the evolutionary plasticity of Musaceae RPD3/HDA1 HDACs and defines MaHDAC3 as an epigenetic brake on carbon metabolism, offering a promising target for extending fruit shelf life. 2026-09-21T00:00:00Z Dataset Dataset 10.1021/acs.jafc.6c06018.s001 https://figshare.com/articles/dataset/A_Pangenome_Analysis_of_the_RPD3_HDA1-Type_Histone_Deacetylase_HDAC_Family_in_Musaceae_Unveils_MaHDAC3_as_an_Epigenetic_Repressor_of_Fruit_Ripening/33954647 CC BY-NC 4.0