2026-10-14T17:53:53Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33954546 2026-09-21T13:44:20Z category_4 category_8 category_13 category_16 category_39 category_734 category_62 category_69 portal_63 item_type_3 month_year_09_2026

From Planktonic to Sedentary Lifestyle: Molecular Dissection of the Establishment and Maintenance of Mycobacterial Biofilm Himalaya Naik (25083753) Rohit Satardekar (25083756) Raju Mukherjee (1667161) Vikas Jain (586535) Biochemistry Microbiology Genetics Physiology Ecology Biological Sciences not elsewhere classified Marine Biology Inorganic Chemistry quantitative mass spectrometry based proteome examination bacteria embedded within mature (∼ 5 transcriptomics analysis showed mycobacterial biofilm physiology initial (∼ 2 mature biofilm stages mycobacterial biofilm establishment biofilm stages mature biofilms eps showed sedentary lifestyle related genes oxidative stress msm </ molecular dissection molecular adaptations model organism metabolic shifts marked alteration including upregulation including production eps ). energy metabolism day old complex aggregation combining transcriptomic clear differences Biofilm represents a complex aggregation of bacteria embedded within a self-produced extracellular polymeric substance (EPS). We investigated the characteristics of mycobacterial biofilm using Mycobacterium smegmatis (<i>Msm</i>) as a model organism. By combining transcriptomic (RNA-seq) and proteomic (LC-MS) analyses, the research captures dynamic changes during the establishment and maturation of the biofilm. Transcriptomics analysis showed a distinct gene expression profile as compared to its planktonic form. Interestingly, clear differences were seen between initial (∼2-day old) and mature (∼5-day old) biofilm stages, highlighting phasic gene expression throughout biofilm development. Marked alteration in oxidative stress-related genes and energy metabolism from ATP to NADH was observed. Furthermore, quantitative mass spectrometry-based proteome examination of EPS showed an abundance of cytoplasmic proteins present differentially between initial and mature biofilm stages. Pathway enrichment revealed enhanced oxidative stress responses and metabolic shifts in mature biofilms, including upregulation of NADH dehydrogenase and downregulation of ATP synthase, indicating altered energy metabolism. Our findings thus provide insights into the molecular adaptations, including production of mycofactocin, occurring during mycobacterial biofilm establishment and maturation, and advance our understanding of mycobacterial biofilm physiology. 2026-09-21T00:00:00Z Dataset Dataset 10.1021/acsomega.6c07807.s002 https://figshare.com/articles/dataset/From_Planktonic_to_Sedentary_Lifestyle_Molecular_Dissection_of_the_Establishment_and_Maintenance_of_Mycobacterial_Biofilm/33954546 CC BY-NC 4.0