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oai:figshare.com:article/33951577 2026-09-21T09:21:37Z category_1280 portal_316 item_type_3 month_year_09_2026

Data Sheet 2_Plasma metabolomics reveals metabolic signatures associated with Lactobacillus reuteri supplementation in ewes.pdf Ivory Smith (25082137) Oluwatobi Fijabi (24732220) Brou Kouakou (14133216) Adel R. Moawad (4967119) Ibukun M. Ogunade (7822001) Olanrewaju B. Morenikeji (7319321) Sunday Peters (8456286) Modoluwamu Idowu (11957168) Clint Loest (25082140) Taylor Sidney (11957174) Godstime Taiwo (11957165) Farm Management, Rural Management and Agribusiness biomarkers ewes feed efficiency immunity Lactobacillus reuteri (LR) metabolome <p>This study investigated the effects of Lactobacillus reuteri (LR) supplementation on the performance and plasma metabolome of ewes using untargeted metabolomics. Eighteen Katahdin ewes (average BW = 26.32 kg) were randomly assigned to either a basal diet (CON; n = 9) or the basal diet supplemented with 10 g per head per day of freeze dried LR (n = 9). Performance data and blood samples were collected weekly over a 35-d period. Initial body weight and daily feed offered did not differ between groups (P > 0.05). LR supplemented ewes had greater total weight gain, average daily gain (ADG), and final body weight and a lower feed to gain ratio than CON ewes (all P < 0.05). Plasma samples collected across the five weekly timepoints were pooled in equal volumes per ewe to generate one cumulative composite sample per animal (n = 18) and were analyzed by chemical isotope labeling liquid chromatography mass spectrometry. Among 1,003 metabolites detected, 27 were differentially abundant (P < 0.05); 9 were elevated in LR, including cyanamide, aspartic acid, L-homoserine, 1-pyrroline-4-hydroxy-2-carboxylic acid, and 3-hydroxypicolinic acid, while 18 were elevated in CON, including 9-oxononanoic acid, isomer 2 of sebacic acid, hydroxylated lecithin, L-fucose, tyramine, and nocardicin A/isonocardicin A. Pathway analysis identified cysteine and methionine metabolism, tryptophan metabolism, primary bile acid biosynthesis, and lysine degradation as enriched in LR ewes (P < 0.05, topology impact > 0.10). Biomarker analyses identified six metabolites, cyanamide, β-alanine, L-ornithine/D-ornithine, hydroxylated lecithin, 9-oxononanoic acid, and isomer 2 of sebacic acid, with sufficient sensitivity and specificity (P < 0.05, Area Under the Curve (AUC) > 0.87) to serve as putative biomarkers of LR supplementation. Plasma cyanamide correlated positively (ρ = 0.67, P < 0.01) and hydroxylated lecithin negatively (ρ = -0.75, P < 0.001) with average daily gain, with hydroxylated lecithin also linked to feed: gain (ρ = 0.68, P < 0.01), identifying it as the strongest single biomarker of the growth and feed efficiency phenotype. The broad metabolite depletion in LR ewes is consistent with enhanced peripheral substrate utilization, indicating that these findings suggest that LR supplementation may improve performance through alterations in amino acid, redox, and immunomodulatory pathways. Validation in larger, multi-breed cohorts is warranted.</p> 2026-09-21T09:21:37Z Dataset Dataset 10.3389/fanim.2026.1926978.s004 https://figshare.com/articles/dataset/Data_Sheet_2_Plasma_metabolomics_reveals_metabolic_signatures_associated_with_Lactobacillus_reuteri_supplementation_in_ewes_pdf/33951577 CC BY 4.0