2026-10-08T11:45:21Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33951214 2026-10-01T14:45:44Z category_24184 category_24634 portal_918 item_type_3 month_year_10_2026

Research data for <b>Paired sequencing of IgA-bound bacteria</b> Logan Sauers (25081504) Bioinformatic methods development Immunology not elsewhere classified Microbiome Immunoglobulin A Metagenomics Metatranscriptomics <p dir="ltr">All bioinformatics outputs related to "Paired sequencing of IgA-bound bacteria reveals widespread associations between adaptive immunity and gut microbiome gene expression" which are required to recreate the statistical analysis in the manuscript.</p><p dir="ltr">Data were generated from IgA-sorted (FACS) gut microbiota of IgMi mice, which produce monoclonal IgA. Positive (IgA-bound) and negative (IgA-unbound) bacterial fractions were subjected to paired metagenomic and metatranscriptomic sequencing. Full details of the MAG assembly pipeline and bioinformatics processing are available in the manuscript and at <a href="https://github.com/SauersALogan/PIg-Seq" target="_blank">SauersALogan/PIg-Seq</a>. </p><p dir="ltr">This deposit contains the following files:</p><ol><li>subsampled_dna_abundance.csv — Abundance of each bin from metagenomic sequencing after subsampling reads to equal depth across samples.</li><li>subsampled_rna_activity.csv — Abundance of each bin from metatranscriptomic sequencing after subsampling reads to equal depth across samples.</li><li>DNA_gene_count_controlled_subsampled.csv — Count of each gene from metagenomic sequencing after subsampling reads to equal depth across samples. Used for random forest.</li><li>RNA_gene_count_controlled_subsampled.csv — Count of each gene from metatranscriptomic sequencing after subsampling reads to equal depth across samples. Used for random forest.</li><li>DNA_features_controlled.csv — Count of each gene from metagenomic sequencing.</li><li>RNA_features_controlled.csv — Count of each gene from metatranscriptomic sequencing.</li><li>Pos_rel_abund_30-09-2025.csv — Relative abundance of each bin per sample in the positive (IgA-bound) fraction, for IgA score calculation.</li><li>Neg_rel_abund_30-09-2025.csv — Relative abundance of each bin per sample in the negative (IgA-unbound) fraction, for IgA score calculation.</li><li>Taxonomy_30-09-2025.csv — Taxonomic assignment of each bin.</li><li>Bin_number_to_taxonomy.csv — Map linking bin number to taxonomic assignment.</li><li>metadata_30-09-2025.csv — Sample metadata.</li><li>parsed_gff.txt — Bakta annotation of each gene identified in the bins.</li></ol><p dir="ltr">Raw sequencing reads will be deposited under PRJEB127118 with the ENA.</p> 2026-10-01T14:45:44Z Dataset Dataset 10.48420/33951214.v1 https://figshare.com/articles/dataset/Research_data_for_b_Paired_sequencing_of_IgA-bound_bacteria_b_/33951214 CC0