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oai:figshare.com:article/33949984 2026-09-21T05:36:43Z category_8 portal_316 item_type_3 month_year_09_2026

Data Sheet 1_Optimization of extraction conditions for total flavonoids from Clerodendrum cyrtophyllum Turcz. and its antibacterial activity and mechanism against Staphylococcus aureus.pdf Yue Hu (201714) Wenwen Luo (1567732) Chengjian Tan (25080562) Guoshun Pei (486323) Microbiology antibacterial activity antibacterial mechanism Clerodendrum cyrtophyllum Turcz. component analysis flavonoids response surface optimization Staphylococcus aureus Introduction<p>Clerodendrum cyrtophyllum Turcz., a traditional Chinese medicinal plant, exhibits broad bioactivity. Nevertheless, the flavonoid constituents conferring anti-Staphylococcus aureus (S. aureus) activity and their mechanistic basis remain to be elucidated.</p>Methods<p>Total flavonoid extraction from Clerodendrum cyrtophyllum Turcz. (TFC) was first evaluated by single-factor experiments to screen key variables and subsequently optimized by a Box–Behnken design (BBD) within response surface methodology (RSM). followed by purification with D101 macroporous resin. Liquid chromatography–tandem mass spectrometry (LC–MS/MS) characterized the chemical profile. Antibacterial activity against S. aureus was assessed via inhibition zones, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and growth curves. Mechanisms were explored by evaluating cell surface hydrophobicity (CSH), extracellular alkaline phosphatase (AKP), leakage of nucleic acids/proteins, membrane potential, ATP levels, reactive oxygen species (ROS) content, and morphological alterations.</p>Results<p>The optimal extraction conditions for TFC were determined as 70% ethanol as the solvent, an extraction time of 102 min, a temperature of 64 °C, and a solid–liquid ratio of 1:25 (g: mL), resulting in a yield of 6.48% ± 0.58. After purification, the purity of TFC was increased to 76%, and preliminary analysis identified 85 flavonoid constituents. TFC exhibited inhibition zone diameters of 13 ± 0.45 mm, 16 ± 0.38 mm, and 20 ± 0.45 mm at concentrations of 50, 100, and 150 mg/mL, respectively, with MIC and MBC values of 0.781 mg/mL and 1.562 mg/mL. Notably, TFC effectively inhibited the growth of S. aureus within 24 h. Mechanistic investigations revealed that TFC treatment increased S. aureus CSH and decreased the membrane potential, leading to the leakage of intracellular nucleic acids and proteins, indicating damage to S. aureus cells, which was further confirmed by SEM. Furthermore, TFC induced significant intracellular ATP depletion, decreased intracellular nucleic acids and protein contents, and triggered excessive ROS production.</p>Conclusion<p>This study optimized the extraction conditions of TFC and successfully obtained TFC rich in multiple flavonoid constituents. TFC exerts potent inhibitory effects on S. aureus by disrupting the integrity of cell walls and membranes, depleting intracellular energy reserves, decreasing the contents of intracellular nucleic acids and proteins, and inducing intracellular oxidative stress.</p> 2026-09-21T05:36:43Z Dataset Dataset 10.3389/fmicb.2026.1961127.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Optimization_of_extraction_conditions_for_total_flavonoids_from_Clerodendrum_cyrtophyllum_Turcz_and_its_antibacterial_activity_and_mechanism_against_Staphylococcus_aureus_pdf/33949984 CC BY 4.0