2026-10-10T14:41:35Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33949504 2026-09-21T05:28:13Z category_696 portal_316 item_type_3 month_year_09_2026

Supplementary file 3_Recurrent antibody repertoire features associated with six selected human IGHV genes: V(D)J usage, heavy-light pairing, and CDRH3 properties.zip Mengmeng Zhang (1507543) Daojing Wang (1310832) Wenwen Xi (25080325) Huihui Jia (1710019) Beifen Shen (78998) Yan Wen (416285) Jing Wang (6206297) Jiannan Feng (367523) Genetic Immunology IGHV genes V(D)J recombination VH-VL pairing CDRH3 physicochemical features Observed Antibody Space paired antibody repertoire Introduction<p>Several human IGHV genes recur in infection, autoreactivity, B-cell malignancy, and antibody-discovery studies, but their downstream V(D)J usage, observed heavy-light pairing, and CDRH3 properties have rarely been examined together in paired human repertoires.</p>Methods<p>We assembled 3,003,127 paired heavy- and light-chain records from public Observed Antibody Space (OAS) source files. After quality control, gene-level standardization, and exact-clonotype collapsing, the primary six-gene analysis included 712,143 exact clonotypes assigned to IGHV1-2, IGHV1-69, IGHV3-21, IGHV3-23, IGHV3-30, or IGHV4-34.</p>Results<p>Marginal V-D effects were generally modest, whereas recurrent V-conditioned D-J modules and IGHV-associated VH-VL enrichment or depletion were repeatedly observed across OAS study groups in productive expressed repertoires. Several VH-VL directions persisted across studies and metadata-defined immune contexts, although two pairs were context-sensitive. External comparison with PairedAbNGS supported 8 of 10 evaluable key VH-VL directions and the broad IGHV-associated CDRH3 profiles. IGHV4-34 showed a study-robust, cross-resource positive shift in CDRH3 side-chain charge score.</p>Discussion<p>Together, these analyses provide a study-aware reference for V-conditioned D-J usage, VH-VL associations, and CDRH3 properties across six selected IGHV backgrounds, while defining the limits imposed by immune-context heterogeneity and productive-repertoire sampling.</p> 2026-09-21T05:28:13Z Dataset Dataset 10.3389/fimmu.2026.1888170.s002 https://figshare.com/articles/dataset/Supplementary_file_3_Recurrent_antibody_repertoire_features_associated_with_six_selected_human_IGHV_genes_V_D_J_usage_heavy-light_pairing_and_CDRH3_properties_zip/33949504 CC BY 4.0