2026-10-13T08:49:51Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33948925 2026-09-21T04:28:36Z category_696 portal_316 item_type_3 month_year_09_2026

Supplementary file 1_Myeloid EGR1 contributes to Th17/Treg imbalance during acute liver allograft rejection in a rat model.docx Yaming Ding (25079836) Jie Wang (16762) Shulin Li (222450) Guoxiang Shen (2102542) Lihong Chen (12138) Genetic Immunology acute rejection (AR) early growth response protein 1 (EGR1) liver transplantation myeloid cells single-cell RNA sequencing Th17/Treg balance Background<p>Ischemia-reperfusion injury (IRI) and acute rejection (AR) limit liver transplantation success. Early growth response 1 (EGR1) mediates stress and inflammation, yet its cellular distribution and potential involvement in adaptive alloimmunity during distinct post-transplant stages remain unclear.</p>Methods<p>A rat orthotopic liver transplantation model was used to evaluate graft injury and EGR1 spatiotemporal expression during IRI (6–24 h) and AR (5–10 d). Public scRNA-seq data and virtual knockout analysis were used to assess the cellular distribution and potential regulatory role of Egr1. EGR1-overexpressing bone marrow-derived dendritic cells (BMDCs) were co-cultured with CD4⁺ T-cells to assess cytokine secretion, proliferation, and shifted Th17/Treg differentiation.</p>Results<p>Both syngeneic and allogeneic grafts exhibited early IRI. By days 5–10, allografts developed AR with elevated liver enzymes and Banff scores. EGR1 rose transiently during IRI but re-escalated exclusively in allografts during AR, localizing to portal infiltrates. scRNA-seq showed preferential Egr1 expression in graft myeloid cells, while virtual knockout suggested immune-related regulatory effects. EGR1-associated APC activation and Th17-associated responses were observed in rejecting allografts. EGR1 overexpression in BMDCs promoted pro-inflammatory cytokine secretion, T-cell proliferation, and altered Th17/Treg-associated responses.</p>Conclusion<p>EGR1 contributes to liver allograft rejection by modulating myeloid APC responses and Th17/Treg-associated T-cell responses, highlighting its potential as a therapeutic target for AR.</p> 2026-09-21T04:28:36Z Dataset Dataset 10.3389/fimmu.2026.1901189.s001 https://figshare.com/articles/dataset/Supplementary_file_1_Myeloid_EGR1_contributes_to_Th17_Treg_imbalance_during_acute_liver_allograft_rejection_in_a_rat_model_docx/33948925 CC BY 4.0