2026-10-13T20:10:16Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33948778 2026-09-21T04:26:29Z category_90 portal_316 item_type_3 month_year_09_2026

Data Sheet 1_CRISPR/Cas9-mediated disruption of OsPLDβ1 enhances salt stress tolerance in rice through modulation of antioxidant defense.pdf Sangeetha Karippadakam (25079749) V. Mohan M. Achary (5432216) Dalia Vishnudasan (25079752) Hemangini Parmar (25079755) Alexander Balamurugan (14287184) Mallesham Bulle (25079758) Ganesan Prakash (12660343) Malireddy K. Reddy (11743874) Genetic Engineering OsPLDβ1 CRISPR/Cas9 rice salt stress salinity tolerance antioxidant defense reactive oxygen species <p>Soil salinity is a critical abiotic stress that severely constrains rice productivity and threatens global food security. Phospholipase D (PLD) enzymes play pivotal roles in plant stress signaling; however, the specific function of OsPLDβ1 in rice salt stress responses remains unclear. We here investigated the role of OsPLDβ1 using knockout mutants to elucidate its regulatory function under saline conditions. Ospldβ1-mutant lines exhibited substantially improved salt tolerance in contrast to wild-type plants. Improved tolerance was associated with elevated reactive oxygen species (ROS) scavenging capacity, indicating improved oxidative stress management. Furthermore, the mutant lines displayed increased expression of ion transport and homeostasis genes and maintained photosynthetic efficiency and agronomic performance. Collectively, these physiological and molecular responses demonstrate that OsPLDβ1 negatively regulates salt stress tolerance in rice. This research highlights the role of OsPLDβ1 in salt tolerance in rice and lays the groundwork for developing salinity-resistant crop varieties through genetic manipulation.</p> 2026-09-21T04:26:29Z Dataset Dataset 10.3389/fgeed.2026.1904040.s001 https://figshare.com/articles/dataset/Data_Sheet_1_CRISPR_Cas9-mediated_disruption_of_OsPLD_1_enhances_salt_stress_tolerance_in_rice_through_modulation_of_antioxidant_defense_pdf/33948778 CC BY 4.0