2026-10-06T10:28:06Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33946489 2026-09-30T10:03:30Z category_23890 item_type_3 month_year_09_2026

Dataset for miR-433-3p protects against cadmium-induced intestinal injury via targeting MAPK8 to repress the JNK-JUN signaling pathway yuanwei wan (25075915) Zhimin Wu (25079120) Bingchen Wang (25079126) Huimin Zhang (18421069) Shuo Yan (18421060) Ting Wang (25079130) Xinyue Cheng (25079136) Zimeng Ma (25079138) Siqin Bao (18421094) Xihe Li (18421091) Jiuzhi Xu (25079143) Yongli Song (18421097) Animal growth and development Hu sheep; Cadmium; miR-433-3p; MAPK8; JNK-JUN signaling; Intestinal inflammation Cadmium (Cd), a widespread environmental pollutant with potent bioaccumulation and persistent toxicity to animals, primarily targets the gastrointestinal tract. Although Cd induces intestinal inflammation, the molecular mechanisms governing this pathological process, particularly post-transcriptional regulatory events, remain largely unclear. Here we constructed a Cd-exposed Hu sheep model and found that Cd accumulation caused significant intestinal inflammatory injury, elevated pro-inflammatory cytokine levels and disrupted intestinal homeostasis. Integrated transcriptomics identified miR-433-3p as a candidate regulatory miRNA markedly downregulated upon Cd treatment. Functional assays demonstrated that miR-433-3p overexpression substantially alleviated Cd-induced inflammatory responses, whereas miR-433-3p inhibition aggravated inflammation. Pathway enrichment identified MAPK signaling as the key downstream cascade modulated by miR-433-3p. Mechanistically, Cd activated the JNK/MAPK8-JUN pathway, while miR-433-3p restoration suppressed this cascade. Bioinformatics prediction combined with dual-luciferase reporter assays verified MAPK8 as a direct target of miR-433-3p. In Cd-challenged mice, in vivo administration of miR-433-3p mimics significantly repressed JNK/MAPK8-JUN signaling activation, thereby ameliorating Cd-provoked intestinal damage and inflammatory responses. Collectively, our findings <a href="" target="_blank">reveal a previously unrecognized post-transcriptional regulatory mechanism</a>: Cd-driven miR-433-3p reduction de-represses MAPK8 to hyperactivate the JNK-JUN inflammatory cascade, where miR‑433‑3p serves as a critical negative regulator (molecular brake) of this inflammatory axis. Our study provides new insights into Cd-induced intestinal injury and suggests miR-433-3p as a potential therapeutic target against heavy-metal-associated intestinal toxicity. 2026-09-29T00:00:00Z Dataset Dataset 10.6084/m9.figshare.33946489.v1 https://figshare.com/articles/dataset/Dataset_for_b_miR-433-3p_protects_against_cadmium-induced_intestinal_injury_via_targeting_b_b_i_MAPK8_i_b_b_to_repress_the_JNK-JUN_signaling_pathway_b_/33946489 CC BY 4.0