2026-10-13T17:44:54Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33945718 2026-09-20T17:27:01Z category_4 category_12 category_13 category_14 category_16 category_46 category_734 category_61 category_128 category_132 category_135 portal_87 item_type_3 month_year_09_2026

Immune cell enrichment characteristics and related key genes in intrauterine adhesion: insights from RNA-sequencing analysis Xue Feng (147580) Lili Yu (149552) Biochemistry Cell Biology Genetics Molecular Biology Physiology Immunology Biological Sciences not elsewhere classified Developmental Biology Hematology Infectious Diseases Computational Biology Immune cell enrichment key genes intrauterine adhesion bioinformatics analysis <p>Intrauterine adhesion (IUA) represents a prevalent cause of persistent infertility and continues to be a significant unresolved challenge in reproductive medicine. However, its full pathogenic mechanisms remain incompletely elucidated. We retrieved endometrial RNA-sequencing data from IUA patients and normal controls in the Gene Expression Omnibus (GEO) database, and screened 586 differentially expressed genes (DEGs) in the endometrium of IUA patients using the R package limma. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and gene set enrichment analysis (GSEA) revealed activation of immune-related pathways, including chronic inflammatory response, complement activation, and complement and coagulation cascades in IUA. Further single-sample gene set enrichment analysis (ssGSEA)-based relative immune cell enrichment analysis revealed decreased enrichment scores of activated CD4 T cells and type 2 T helper cells, alongside increased enrichment scores of effector memory CD8 T cells, macrophages, mast cells, natural killer cells, and T follicular helper cells in IUA patients, indicating their potential involvement in IUA pathogenesis. A protein–protein interaction (PPI) network was constructed, identifying 10 hub genes associated with IUA: <i>CDK1</i>, <i>CCNB1</i>, <i>CCNA2</i>, <i>CENPA</i>, <i>BUB1</i>, <i>AURKB</i>, <i>KIF11</i>, <i>BUB1B</i>, <i>CDC20</i>, and <i>TOP2A</i>. The expression patterns of these genes were further validated in two independent external IUA cohorts, supporting a potential correlative link between these genes and IUA pathogenesis. Our findings provide exploratory insights and potential candidate molecules for subsequent mechanistic studies of IUA onset and progression.</p> 2026-09-20T17:27:01Z Dataset Dataset 10.6084/m9.figshare.33945718.v1 https://figshare.com/articles/dataset/Immune_cell_enrichment_characteristics_and_related_key_genes_in_intrauterine_adhesion_insights_from_RNA-sequencing_analysis/33945718 CC BY 4.0