2026-10-12T20:48:11Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33942734 2026-09-19T14:22:57Z category_1 category_915 category_12 category_13 category_15 category_272 category_873 category_734 category_931 category_64 category_69 portal_63 item_type_2 month_year_09_2026

Region-Resolved Label-Free Raman Profiling of Perturbation Responses in Intestinal Organoids Ruhao Zhang (6021550) Biao Chen (795331) Xiaowen Cheng (230980) Yilin Lu (1747810) Tong Li (206968) Shengmin Xu (2147704) Lijun Wu (152858) Biophysics Physical Sciences not elsewhere classified Cell Biology Genetics Neuroscience Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified Information Systems not elsewhere classified Cancer Inorganic Chemistry physiologically relevant three partial return toward organoid response evaluation detectable differences even destructive analytical methods broad molecular readout studying biological processes associated raman bands associated spectral deviations >- associated band free raman profiling murine intestinal organoids spatially resolved detection associated band spatially resolved spectral responses dynamic processes associated responses associated changes intestinal organoids sensitive raman sensitive detection vitro models using irradiation subtle perturbation spatial organization remain limited recent years primarily reflected perturbation responses observed dose mild perturbation microenvironmental features longitudinal monitoring evaluating perturbation efficient workflow early decrease corresponding changes coordinated variations controlled perturbation control level c </ based workflow 72 h 2 h 1656 cm 1583 cm 1092 cm In recent years, organoids have emerged as physiologically relevant three-dimensional in vitro models for studying biological processes as they can better recapitulate tissue architecture, spatial organization, and microenvironmental features. However, current organoid studies still rely predominantly on destructive analytical methods and remain limited in spatially resolved detection, broad molecular readout, and sensitive detection of subtle perturbation-associated changes. We established a label-free, spatially resolved, and sensitive Raman-based workflow for evaluating perturbation-associated responses in intestinal organoids. Using irradiation as a controlled perturbation, we observed dose- and time-dependent Raman spectral changes in murine intestinal organoids, with detectable differences even under mild perturbation. These spectral responses were primarily reflected in coordinated variations of the 785 and 1092 cm^–1 nucleic-acid-associated Raman bands, showing an early decrease at 2 h and a partial return toward the control level at 24 and 72 h, with further support from corresponding changes in the 1656 cm^–1 protein-associated band and the 1583 cm^–1 cytochrome <i>c</i>-associated band. Notably, region-resolved Raman analysis revealed spatial heterogeneity, showing that the stem-cell-enriched region exhibited larger perturbation-associated spectral deviations than the differentiated-cell-associated region. Together, these findings support a practical, sensitive, spatially resolved, and resource-efficient workflow for organoid response evaluation, with potential for longitudinal monitoring of dynamic processes. 2026-09-19T00:00:00Z Dataset Media 10.1021/acs.langmuir.6c04180.s002 https://figshare.com/articles/media/Region-Resolved_Label-Free_Raman_Profiling_of_Perturbation_Responses_in_Intestinal_Organoids/33942734 CC BY-NC 4.0