2026-10-13T13:50:47Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/33939858
2026-09-18T17:53:49Z
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<p>Primer sequences.</p>
Elliot Lloyd (12629927)
Michael Cremin (17667123)
Emmy Tay (25073577)
Kristina Sanchez (25073580)
Jungjae Park (8505789)
YuanYuan Lei (25073583)
Melanie G. Gareau (11268202)
Colin Reardon (4285078)
Medicine
Microbiology
Physiology
Immunology
Infectious Diseases
regulate key aspects
reduced chemokine production
cause significant morbidity
sensory neuropeptides encoded
colonic bacterial burden
numerous organ systems
>< sup ><
host protective effect
citrobacter rodentium </
tac1 deficiency reduces
activate substance p
reduced immune response
encoded sensory neurotransmitters
specific immune cells
enteric bacterial infection
immune systems
sensory neurons
immune response
bacterial enumeration
host response
></ sup
tac1 </
well appreciated
unique targets
receptor signaling
precise contribution
neuroimmune circuits
mortality throughout
microbial stimuli
may serve
localized release
inflammatory cytokines
effective control
detect inflammatory
decreased expression
bidirectional communication
assessed using
<div>
<p>Background</p><p>Infection with enteric bacterial pathogens continues to cause significant morbidity and mortality throughout the world. Effective control of these infections requires coordinated immune responses that are shaped in part by bidirectional communication between the nervous and immune systems. Sensory neurons are now well appreciated to detect inflammatory or microbial stimuli in numerous organ systems, including the gastrointestinal (GI) tract, and for eliciting the recruitment or activation of specific immune cells. Central to the host protective effect of these neuroimmune circuits is the localized release of neurotransmitters that activate substance P (SP) receptor signaling. However, the precise contribution of sensory neuropeptides encoded by the <i>Tac1</i> gene, such as SP, in the host response to enteric infection remains incompletely understood.</p>
<p>Methods</p><p>The role of the Tac1-encoded sensory neurotransmitters in the host response to <i>Citrobacter rodentium</i> was assessed using <i>Tac1</i><sup><i>-/-</i></sup> and wildtype (WT) mice. Infection and inflammation were determined by bacterial enumeration, histopathology, qPCR, and flow cytometry.</p>
<p>Results</p><p><i>Tac1</i><sup>-/-</sup> mice had significantly reduced pathogen shedding and colonic bacterial burden, accompanied by decreased expression of inflammatory cytokines and chemokines compared to WT controls. In accordance with reduced chemokine production, we observed reduced colonic recruitment of neutrophils, monocytes, and IFNγ- and IL-17A-producing T-cells in <i>Tac1</i><sup><i>-/-</i></sup> compared with WT mice. This reduced immune response was associated with markedly reduced colonic histopathology.</p>
<p>Conclusions</p><p>Sensory neuropeptides encoded by <i>Tac1</i> regulate key aspects of the immune response to enteric bacterial infection and may serve as unique targets in the treatment of enteric disease.</p></div>
2026-09-18T17:51:24Z
Dataset
Dataset
10.1371/journal.ppat.1014587.t001
https://figshare.com/articles/dataset/_p_Primer_sequences_p_/33939858
CC BY 4.0