2026-10-12T03:14:29Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33939640 2026-09-18T17:50:51Z category_4 category_7 category_13 category_14 category_21 category_272 category_873 category_734 category_931 portal_5 item_type_3 month_year_09_2026

<p>Concentration and purity of gDNA extracted from different adult zebrafish tissues using the Chelex‑100 resin method and a commercial kit.</p> Wei Feng (225281) Boya Li (3147633) Yiqiong Niu (25073545) Qiannan Xu (230981) Xiandun Zhai (23757735) Biochemistry Medicine Genetics Molecular Biology Biotechnology Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified Information Systems not elsewhere classified suggesting reduced sensitivity operationally simple alternative danio rerio </ consistent amplicon quality 56 ° c 02 ), indicating 00 per sample routine molecular applications 5 ± 22 84 – 2 2 ± 3 time pcr assays div >< p adult zebrafish tissues 100 ° c 36 – 1 estimated reagent cost routine gdna extraction genomic dna extraction genomic dna effective extraction zebrafish (< tested tissues qpcr applications 5 ng target fragments systematically optimized pure templates proteinase k proteinaceous impurities practical challenge particularly suitable optimized protocol involving incubation generally higher consistently lower column purification biomedical research based method based genotyping a280 ratios <$ 0 15 min 10 min <p>Concentration and purity of gDNA extracted from different adult zebrafish tissues using the Chelex‑100 resin method and a commercial kit.</p> 2026-09-18T17:50:41Z Dataset Dataset 10.1371/journal.pone.0358591.t001 https://figshare.com/articles/dataset/_p_Concentration_and_purity_of_gDNA_extracted_from_different_adult_zebrafish_tissues_using_the_Chelex_100_resin_method_and_a_commercial_kit_p_/33939640 CC BY 4.0