2026-10-14T19:28:29Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33939117 2026-09-18T17:45:52Z category_7 category_8 category_12 category_13 category_14 category_16 category_39 category_46 category_734 category_61 category_135 portal_5 item_type_3 month_year_09_2026

<p>Underlying values.</p> Tal Gordon (10653863) Tom Levy (4708123) Chester Jiamu Yu (25073469) Benyamin Rosental (161422) Lauren Lubeck (25073472) Lucia Manni (558352) Irving L. Weissman (9758181) Ayelet Voskoboynik (558357) Medicine Microbiology Cell Biology Genetics Molecular Biology Physiology Ecology Immunology Biological Sciences not elsewhere classified Developmental Biology Computational Biology upper fragments fail reveal striking differences may inform strategies enhance tissue repair combining flow cytometry ciona robusta </ specific signaling cues spatially distinct environments local signals regulate shared injury response div >< p divergent regenerative outcomes signaling environments shaping outcomes local expression well understood unique model transcriptional dynamics solitary ascidian robust proliferative related factors regenerative contexts regenerative capacity mediated regeneration instead depends highlighting principles findings show fate mapping <div><p>Many tissues harbor quiescent stem cells that activate after injury, yet how local signals regulate this transition is not well understood. The solitary ascidian <i>Ciona robusta</i> provides a unique model, as bottom body fragments regenerate while upper fragments fail to do so. By comparing these regenerative and non-regenerative contexts, we reveal striking differences in transcriptional dynamics and signaling environments. Combining flow cytometry, scRNA-seq, transplantation, and fate mapping, we identified a candidate stem cell population with robust proliferative and differentiation potential following transplantation. However, regenerative capacity does not simply reflect stem cell abundance, but instead depends on region-specific signaling cues. Local expression of metabolic, immune and differentiation-related factors further underscores the importance of spatially distinct environments in shaping outcomes. Our findings show how a shared injury response is associated with divergent regenerative outcomes, highlighting principles that may inform strategies to enhance tissue repair in other systems.</p></div> 2026-09-18T17:45:42Z Dataset Dataset 10.1371/journal.pbio.3004000.s006 https://figshare.com/articles/dataset/_p_Underlying_values_p_/33939117 CC BY 4.0