2026-10-12T20:05:34Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33937336 2026-09-18T17:23:44Z category_1 category_4 category_8 category_12 category_13 category_14 category_46 category_734 category_62 category_128 category_134 portal_5 item_type_3 month_year_09_2026

<p>Ppp1r3g interacts with 14-3-3 family proteins.</p> Ruyi Wang (4811667) Man Zhou (196972) Yuxuan Wang (251760) Xudong Hu (354241) Han Zhang (110653) Hong Cao (122228) Biophysics Biochemistry Microbiology Cell Biology Genetics Molecular Biology Immunology Biological Sciences not elsewhere classified Marine Biology Hematology Virology suppress innate immunity innate immune response grass carp reovirus grass carp kidney coagulation cascade activation exacerbate hemorrhage via suppressed gcrv replication gobiocypris rarus </ ctenopharyngodon idella </ study also demonstrated protein phosphatase 1 div >< p study demonstrated via </ gcrv stimulation gcrv ), vivo </ ppp1r3g </ upregulated type type controls suppresses platelet regulatory subunits proteolytic pathway primarily focused pp1c domain pp1 ). lipid metabolism glycogen synthesis findings indicate current research completely elucidated analysis revealed <div><p>Protein phosphatase 1 regulatory subunit 3G (PPP1R3G) is one of the regulatory subunits of protein phosphatase 1 (PP1). Current research on PPP1R3G is primarily focused on glycogen synthesis and lipid metabolism. However, the role of <i>ppp1r3g</i> in the innate immune response of fish has not been completely elucidated. This study demonstrated that <i>ppp1r3g</i> regulates RLR signaling pathway through interactions with Rig-I, Mda5, and Traf6. In parallel, Ppp1r3g inhibits platelet activation by regulating 14-3-3 zeta (14-3-3ζ) and consequently modulating the formation of the 14-3-3ζ-integrin-β3 (Itgb3) complex. <i>In vivo</i> analysis revealed that compared with the wild-type controls, <i>ppp1r3g</i>-null rare minnow (<i>Gobiocypris rarus</i>) and <i>ppp1r3g</i>-chimera grass carp (<i>Ctenopharyngodon idella</i>) exhibited significantly enhanced resistance to grass carp reovirus (GCRV), with markedly alleviated hemorrhagic symptoms post-infection. Furthermore, knockdown of <i>ppp1r3g</i> suppressed GCRV replication and upregulated type I interferon (IFN-1) expression in grass carp kidney (CIK) cells. Additionally, Ppp1r3g binds to the -ESKVFYLKMKGDYYRYL- fragment (EL17) of 14-3-3ζ <i>via</i> the Ser36/37 phosphorylation site located on the PP1c domain. This study also demonstrated that 14-3-3ζ interacts with Itgb3 through this fragment, indicating that Ppp1r3g competes with Itgb3 to bind 14-3-3ζ. Furthermore, Ppp1r3g dose-dependently promoted Itgb3 degradation via a proteolytic pathway. These findings indicate that Ppp1r3g negatively regulates innate antiviral immunity after GCRV stimulation and suppresses platelet and coagulation cascade activation by modulating the interaction between 14-3-3ζ and Itgb3 in fish.</p></div> 2026-09-18T17:23:33Z Dataset Dataset 10.1371/journal.ppat.1014590.s009 https://figshare.com/articles/dataset/_p_Ppp1r3g_interacts_with_14-3-3_family_proteins_p_/33937336 CC BY 4.0