2026-10-10T11:52:50Z https://api.figshare.com/v2/oai

oai:figshare.com:article/33936061 2026-09-18T14:03:51Z category_133 portal_316 item_type_3 month_year_09_2026

Supplementary file 1_Exogenous 24-epibrassinolide enhances chilling tolerance in Phalaenopsis-type Dendrobium through coordinated physiological and transcriptomic responses.docx Xiaoyun Yu (1261878) Xinjie Wei (12356971) Tongyan Yao (25072429) Xiaoyan Luo (484096) Shuangshuang Yi (18286578) Yi Liao (575994) Chonghui Li (445423) Shunjiao Lu (18286575) Plant Biology 24-epibrassinolide (EBR) chilling stress Phalaenopsis-type Dendrobium transcriptomic analysis WGCNA Introduction<p>Phalaenopsis-type Dendrobium is a commercially important tropical orchid that is highly sensitive to chilling, which can cause premature leaf senescence and abscission.</p>Methods<p>We evaluated the protective effect of exogenous 24-epibrassinolide (EBR) and integrated time-resolved RNA-seq with physiological traits previously characterized in the same experimental framework. A concentration of 2.0 mg/L was selected for transcriptomic analysis based on the refined dose-response experiment. WGCNA was performed using 18 EBR-treated libraries. Global GO and KEGG outputs were retained as descriptive overviews, whereas quantitative functional inference was based on module-specific analyses with complete multiple-testing-adjusted statistics.</p>Results<p>EBR delayed leaf yellowing and abscission. Differential-expression analysis revealed time-dependent transcriptional responses during chilling and recovery. WGCNA identified trait-associated modules with contrasting temporal relationships to physiological traits: the pink module was positively associated with MDA and proline and negatively associated with chlorophyll, whereas the antiquewhite4 (AW4) module showed the opposite pattern. Pink-module enrichment emphasized central carbon and energy metabolism, lipid turnover, peroxisomal processes, autophagy, and cellular transport/homeostasis. AW4 GO enrichment was dominated by transmembrane and metabolite transport; no AW4 KEGG pathway remained significant after multiple-testing correction.</p>Discussion<p>These results support an association between EBR treatment and coordinated metabolic, membrane-homeostasis, and stress-response adjustments during chilling and recovery in Phalaenopsis-type Dendrobium.</p> 2026-09-18T14:03:51Z Dataset Dataset 10.3389/fpls.2026.1912617.s001 https://figshare.com/articles/dataset/Supplementary_file_1_Exogenous_24-epibrassinolide_enhances_chilling_tolerance_in_Phalaenopsis-type_Dendrobium_through_coordinated_physiological_and_transcriptomic_responses_docx/33936061 CC BY 4.0