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oai:figshare.com:article/33903881 2026-09-17T17:29:41Z category_8 category_13 category_14 category_21 category_24 category_39 category_734 category_931 category_133 portal_5 item_type_3 month_year_09_2026

<p>A spreadsheet in CSV format (file downloadable separately), providing the raw data for the experimental measurements of each of 123 sequences generated using genomic data from <i>E. coli.</i></p> Alexander D. Duggan (20936492) Matthew P. Newman (18892741) David R. McMillen (2521465) Microbiology Genetics Molecular Biology Biotechnology Evolutionary Biology Ecology Biological Sciences not elsewhere classified Information Systems not elsewhere classified Plant Biology validated genetic parts upstream untranslated regions specific base distributions relatively small number predictive design tools little predictive power inflexible statistical templates fluorescent reporter protein 5 ′- utrs motifs without hard genomic statistics provided escherichia coli </ coding specific motifs uniformly distributed range produce novel sequences 100 unique sequences limosilactobacillus reuteri </ genomic sequence statistics trainable language model synthetic libraries yielded simple generative approach div >< p tuning protein expression modulate translation rates reuteri </ expression tuning genomic data consensus motifs translation rates sequence libraries broad range sequences derived 000 sequences translational tuning window n translation levels thermodynamic predictions tested variants sequenced genome results demonstrate prior knowledge often constrained model probiotic model contexts model bacterium method uses mechanistic rules mechanistic assumptions explicit reference <p>The sheet provides the measured fluorescence from each sequence when a plasmid containing the sequence was transformed into <i>E. coli</i> and when the same plasmid was transformed into <i>L. reuteri.</i></p> <p>(CSV)</p> 2026-09-17T17:29:29Z Dataset Dataset 10.1371/journal.pone.0348455.s008 https://figshare.com/articles/dataset/_p_A_spreadsheet_in_CSV_format_file_downloadable_separately_providing_the_raw_data_for_the_experimental_measurements_of_each_of_123_sequences_generated_using_genomic_data_from_i_E_coli_i_p_/33903881 CC BY 4.0